Transposon genes of rice, transformed plant and method of use

Abstract

A gene of rice has homology to SEQ. ID NO.:2, and a transposase gene of rice has homology to bases 3190-4557 of SEQ. ID NO.:2, or encodes SEQ. ID NO.:4, or variants thereof, plasmids and transformants thereof as well as plasmids and transformants thereof. A transposase has SEQ. ID NO.:4, or a variant thereof. A method of transposing a transposon gene of rice (Oryza sativa) having homology to SEQ. ID NO.:1 comprises transducing a transposon gene of rice having homology to SEQ. ID NO,:2, or a transposase gene of rice encoding a protein comprising SEQ. ID NO.:4 or a variant thereof.

1 . (canceled)  
     
     
         2 . (canceled)  
     
     
         3 . A transposon gene of rice, comprising a nucleotide sequence that is at least 90% homologous to SEQ.ID NO.:2 and has transposon gene activity.  
     
     
         4 . A transposase gene of rice, comprising a nucleotide sequence that is at least 75% homologous to bases 3190 to 4557 of SEQ. ID NO.: 2 and has transposase activity.  
     
     
         5 . A transposase gene encoding a an amino acid sequence comprising SEQ. ID NO.:4, or SEQ. ID NO.:4 wherein one or more amino acids are deleted, substituted or added thereto and has transposase activity.  
     
     
         6 . A transposase comprising SEQ. ID NO.:4, or SEQ. ID NO.:4 wherein one or more amino acids are deleted, substituted or added thereto and has transposase activity.  
     
     
         7 . (canceled)  
     
     
         8 . A plasmid, comprising a promoter and the transposase gene of  claim 4  or a transposase gene encoding an amino acid sequence comprising SEQ. ID NO.:4, or SEQ. ID NO.:4 wherein one or more amino acids are deleted substituted or added thereto and has transposase activity.  
     
     
         9 . (canceled)  
     
     
         10 . (canceled)  
     
     
         11 . (canceled)  
     
     
         12 . A transformant, comprising a host carrying a promoter, and the transposase gene of  claim 4  or a transposase gene encoding an amino acid sequence comprising SEQ. ID NO.:4, or SEQ. ID NO.:4 wherein one or more amino acids are deleted substituted or added thereto and has transposase activity which are transduced.  
     
     
         13 . The transformant of  claim 12 , wherein the host is a plant.  
     
     
         14 . The transformant of  claim 13 , wherein the plant is barley, wheat or maize.  
     
     
         15 . (canceled)  
     
     
         16 . (canceled)  
     
     
         17 . (canceled)  
     
     
         18 . (canceled)  
     
     
         19 . (canceled)  
     
     
         20 . (canceled)  
     
     
         21 . A plasmid comprising the transposon gene of  claim 3 .  
     
     
         22 . A plasmid comprising a promoter and the transposase gene of  claim 5 .  
     
     
         23 . A transformant, comprising a host carrying the transposon gene of  claim 3 , which is transduced.  
     
     
         24 . A transformant, comprising a host carrying a promoter and the transposase gene of  claim 5 , which are transduced.  
     
     
         25 . A method for transposing a nucleotide sequence that is at least 95% homologous to SEQ. ID NO.:I, comprising transducing the transposon gene of  claim 3  to produce a transformant.  
     
     
         26 . The method of  claim 25 , further comprising subjecting the transformant to anther culture or treating it with a chemical agent.  
     
     
         27 . A method for transposing a nucleotide sequence that is at least 95% homologous to SEQ. ID NO.:1, comprising transducing a promoter and the transposon gene of  claim 4  to produce a transformant.  
     
     
         28 . The method of  claim 27 , further comprising subjecting the transformant to anther culture or treating it with a chemical agent.  
     
     
         29 . A method for transposing a nucleotide sequence which is at least 95% homologous to SEQ ID NO: 1, comprising transducing a promoter and the transposon gene of  claim 5  to produce a transformant.  
     
     
         30 . The method of  claim 29 , further comprising subjecting the transformant to anther culture or treating it with a chemical agent.