Method of protecting an animal skin product from metalloproteinase activity

Abstract

Animal skins, pelts, and hides are protected from putrefaction, degradation or deterioration caused by metalloproteinases such as collagenase by applying at least one metalloproteinase inhibitor to a fluid in which the animal skin or hide is dipped or soaked, or applying at least one metalloproteinase inhibitor to a solid surface that directly or indirectly comes into contact with the animal skin or hide

1 . A method of preventing or inhibiting putrefaction, degradation, and/or deterioration of an animal skin or hide, the method comprising applying at least one metalloproteinase inhibitor in a solution to the animal skin or hide, or adding at least one metalloproteinase inhibitor to a fluid in which the animal skin or hide is dipped or soaked, or applying at least one metalloproteinase inhibitor to a solid surface that directly or indirectly comes into contact with the animal skin or hide.  
   
   
       2 . The method of  claim 1  wherein the at least one metalloproteinase inhibitor is a chelator of a divalent metal ion.  
   
   
       3 . The method of  claim 1  wherein the at least one metalloproteinase inhibitor is an inhibitor of a metalloproteinase produced by a microorganism.  
   
   
       4 . The method of  claim 1  wherein the at least one metalloproteinase inhibitor is a collagenase inhibitor.  
   
   
       5 . The method of  claim 1  wherein the at least one metalloproteinase inhibitor is an aminocarboxylic acid or polyaminocarboxylic acid or a salt thereof.  
   
   
       6 . The method of  claim 1  wherein the at least one metalloproteinase inhibitor is ethylenediaminetetraacetic acid or a salt thereof or is ethylenediaminetriacetic acid or a salt thereof.  
   
   
       7 . The method of  claim 1  wherein the at least one metalloproteinase inhibitor is ethylenediaminetetraacetic acid, ethylenediaminetetraacetic acid disodium salt, ethylenediaminetetraacetic acid trisodium salt, ethylenediaminetetraacetic acid tetrasodium salt, ethylenediaminetetraacetic acid dipotassium salt, ethylenediaminetetraacetic acid tripotassium salt, ethylenediaminetetraacetic acid ammonium salt, ethylenediaminetetraacetic acid diammonium salt, or combinations thereof.  
   
   
       8 . The method of  claim 1  wherein the at least one metalloproteinase inhibitor is ethylene glycol-bis(β-aminoethylether)-N,N,N′N′-tetraacetic acid or a salt thereof.  
   
   
       9 . The method of  claim 1  wherein the at least one metalloproteinase inhibitor is ethylene glycol-bis(β-aminoethylether)-N,N,N′N′-tetraacetic acid tetrasodium salt.  
   
   
       10 . The method of  claim 1 , wherein the at least one metalloproteinase inhibitor is S,S′-ethylenediamine disuccinic acid, 1,2-diaminocyclohexene-N,N,N′,N′-tetraacetic acid, N-(2-hydroxyethyl)ethylenediamine-N,N′-triacetic acid, methylglycinediacetic acid, N,N-bis(carboxymethyl)glutamate, ortho-phenanthroline, 8-hydroxyquinoline, amino-tris methylene phosphonic acid, diethylene triamine pentamethylene phosphonic acid, 2-phosphono-1,2,4-butanetricarboxylic acid, hydroxyethylidene-diphosphonic acid, 2-methylpentanediamine tetrakis (methylene phosphonic acid) and 1,2, diaminocyclohexanetetrakis (methylene phosphonic acid), citric acid, a salt of citric acid, gluconic acid, a salt of gluconic acid, cysteine, iodoacetic acid or sodium iodoacetate or combinations thereof.  
   
   
       11 . The method of  claim 1  wherein the at least one metalloproteinase inhibitor is applied to the animal skin or hide in an amount of from about 0.00001% to about 10% by weight based on the animal skin or hide.  
   
   
       12 . The method of  claim 1  wherein the metalloproteinase inhibitor is applied to the animal skin or hide in an amount of from about 0.0001% to about 5% by weight based on the animal skin or hide.  
   
   
       13 . The method of  claim 1  wherein the at least one metalloproteinase inhibitor is applied to the animal skin or hide in an amount of from about 0.001% to about 2% by weight based on the animal skin or hide.  
   
   
       14 . The method of  claim 1  wherein the at least one metalloproteinase inhibitor is applied to the animal skin or hide in a solution by spraying.  
   
   
       15 . The method of  claim 1  wherein the at least one metalloproteinase inhibitor is applied to a fluid that contacts the animal skin or hide so that the at least one metalloproteinase is present in fluid that contacts the animal skin or hide in an amount of from 0.00001% to about 10% by weight of the fluid.  
   
   
       16 . The method of  claim 1  wherein the at least one metalloproteinase inhibitor is applied to a surface that contacts the animal skin or hide by combining the at least one metalloproteinase inhibitor with a fluid medium to form a mixture and applying the mixture to the surface, wherein the at least one metalloproteinase is present in the mixture in an amount of from 0.00001% to about 10% by weight of the mixture.  
   
   
       17 . A method of preventing or inhibiting putrefaction, degradation, and/or deterioration of an animal skin or hide, the method comprising applying at least one polyaminocarboxylic acid, or a salt thereof, in a solution to the animal skin or hide, or adding at least one polyaminocarboxylic acid, or a salt thereof, to a fluid in which the animal skin or hide is dipped or soaked, or applying at least one polyaminocarboxylic acid, or a salt thereof, to a solid surface that directly or indirectly comes into contact with the animal skin or hide.  
   
   
       18 . The method of  claim 17  wherein the at least one polyaminocarboxylic acid is ethylenediaminetetraacetic acid or a salt thereof or is ethylenediaminetriacetic acid or a salt thereof.  
   
   
       19 . The method of  claim 17  wherein the at least one polyaminocarboxylic acid is ethylenediaminetetraacetic acid, ethylenediaminetetraacetic acid disodium salt, ethylenediaminetetraacetic acid trisodium salt, ethylenediaminetetraacetic acid tetrasodium salt, ethylenediaminetetraacetic acid dipotassium salt, ethylenediaminetetraacetic acid tripotassium salt, ethylenediaminetetraacetic acid ammonium salt, or ethylenediaminetetraacetic acid diammonium salt.  
   
   
       20 . The method of  claim 17  wherein the at least one polyaminocarboxylic acid is ethylene glycol-bis(β-aminoethylether)-N,N,N′N′-tetraacetic acid or a salt thereof.  
   
   
       21 . The method of  claim 17  wherein the at least one polyaminocarboxylic acid is ethylene glycol-bis(β-aminoethylether)-N,N,N′N′-tetraacetic acid tetrasodium salt.  
   
   
       22 . The method of  claim 1 , wherein the at least one metalloproteinase inhibitor is S,S′-ethylenediamine disuccinic acid, 1,2-diaminocyclohexene-N,N,N′,N′-tetraacetic acid, N-(2-hydroxyethyl)ethylenediamine-N,N′-triacetic acid, methylglycinediacetic acid, N,N-bis(carboxymethyl)glutamate, ortho-phenanthroline, 8-hydroxyquinoline, amino-tris methylene phosphonic acid, diethylene triamine pentamethylene phosphonic acid, 2-phosphono-1,2,4-butanetricarboxylic acid, hydroxyethylidene-diphosphonic acid, 2-methylpentanediamine tetrakis (methylene phosphonic acid) and 1,2, diaminocyclohexanetetrakis (methylene phosphonic acid), citric acid, a salt of citric acid, gluconic acid, a salt of gluconic acid, cysteine, iodoacetic acid or sodium iodoacetate or combinations thereof.  
   
   
       23 . The method of  claim 17  wherein the at least one polyaminocarboxylic acid is applied to the animal skin or hide in an amount of from about 0.00001% to about 10% by weight based on the animal skin or hide.  
   
   
       24 . The method of  claim 17  wherein the polyaminocarboxylic acid is applied to the animal skin or hide in an amount of from about 0.0001% to about 5% by weight based on the animal skin or hide.  
   
   
       25 . The method of  claim 17  wherein the at least one polyaminocarboxylic acid is applied to the animal skin or hide in an amount of from about 0.001% to about 2% by weight based on the animal skin or hide.  
   
   
       26 . A method of producing leather comprising steps of detaching an animal skin or hide from a slaughtered animal, curing the animal skin or hide, soaking the cured animal skin or hide, removing flesh and hair from the soaked animal skin or hide, and tanning the animal skin or hide from which the flesh and hair has been removed to form leather, wherein after the step of detaching the animal skin or hide from the slaughtered animal and before the step of tanning the animal skin or hide from which the flesh and hair has been removed to form leather, a solution comprising at least one metalloproteinase inhibitor is applied to the animal skin or hide in a sufficient amount to prevent or inhibit putrefaction, degradation, and/or deterioration of the animal skin or hide.  
   
   
       27 . A composition comprising a dipping or soaking solution having at least one animal skin or hide dipped or soaked therein, wherein the dipping or soaking solution contains at least one metalloproteinase inhibitor.  
   
   
       28 . The composition of  claim 27  wherein the at least one metalloproteinase inhibitor is a chelator of a divalent metal ion.  
   
   
       29 . The composition of  claim 27  wherein the at least one metalloproteinase inhibitor is an inhibitor of a metalloproteinase produced by a microorganism.  
   
   
       30 . The composition of  claim 27  wherein the at least one metalloproteinase inhibitor is a collagenase inhibitor.  
   
   
       31 . The composition of  claim 27  wherein the at least one metalloproteinase inhibitor is an aminocarboxylic acid or polyaminocarboxylic acid or a salt thereof.  
   
   
       32 . The composition of  claim 27  wherein the at least one metalloproteinase inhibitor is ethylenediaminetetraacetic acid or a salt thereof or is ethylenediaminetriacetic acid or a salt thereof.  
   
   
       33 . The composition of  claim 27  wherein the at least one metalloproteinase inhibitor is ethylenediaminetetraacetic acid, ethylenediaminetetraacetic acid disodium salt, ethylenediaminetetraacetic acid trisodium salt, ethylenediaminetetraacetic acid tetrasodium salt, ethylenediaminetetraacetic acid dipotassium salt, ethylenediaminetetraacetic acid tripotassium salt, ethylenediaminetetraacetic acid ammonium salt, ethylenediaminetetraacetic acid diammonium salt, or combinations thereof.  
   
   
       34 . The composition of  claim 27  wherein the at least one metalloproteinase inhibitor is ethylene glycol-bis(β-aminoethylether)-N,N,N′N′-tetraacetic acid or a salt thereof.  
   
   
       35 . The composition of  claim 27  wherein the at least one metalloproteinase inhibitor is ethylene glycol-bis(β-aminoethylether)-N,N,N′N′-tetraacetic acid tetrasodium salt.  
   
   
       36 . The composition of  claim 27 , wherein the at least one metalloproteinase inhibitor is S,S′-ethylenediamine disuccinic acid, 1,2-diaminocyclohexene-N,N,N′,N′-tetraacetic acid, N-(2-hydroxyethyl)ethylenediamine-N,N′-triacetic acid, methylglycinediacetic acid, N,N-bis(carboxymethyl)glutamate, ortho-phenanthroline, 8-hydroxyquinoline, amino-tris methylene phosphonic acid, diethylene triamine pentamethylene phosphonic acid, 2-phosphono-1,2,4-butanetricarboxylic acid, hydroxyethylidene-diphosphonic acid, 2-methylpentanediamine tetrakis (methylene phosphonic acid) and 1,2, diaminocyclohexanetetrakis (methylene phosphonic acid), citric acid, salt of citric acid, gluconic acid, salt of gluconic acid, cysteine, iodoacetic acid or sodium iodoacetate or combinations thereof.  
   
   
       37 . The composition of  claim 27  wherein the at least one metalloproteinase inhibitor is contained in the dipping or soaking solution in an amount of from about 0.00001% to about 10% by weight based on the at least one animal skin or hide dipped or soaked therein.  
   
   
       38 . The composition of  claim 27  wherein the dipping or soaking solution is a cleaning solution.  
   
   
       39 . The composition of  claim 27  wherein the dipping or soaking solution is a chilling solution having a temperature above freezing and below room temperature.  
   
   
       40 . The composition of  claim 27  wherein the dipping or soaking solution is a curing or pickling solution.  
   
   
       41 . The composition of  claim 27  wherein the dipping or soaking solution is a liming solution.  
   
   
       42 . The composition of  claim 27  wherein the dipping or soaking solution is a deliming solution.  
   
   
       43 . The composition of  claim 27  wherein the dipping or soaking solution is a solution that softens or hydrates the animal skin or hide.  
   
   
       44 . A composition comprising an animal skin or hide having a solution containing at least one metalloproteinase inhibitor applied to at least one surface thereof.  
   
   
       45 . The composition of  claim 44  wherein the at least one metalloproteinase inhibitor is a chelator of a divalent metal ion.  
   
   
       46 . The composition of  claim 44  wherein the at least one metalloproteinase inhibitor is an inhibitor of a metalloproteinase produced by a microorganism.  
   
   
       47 . The composition of  claim 44  wherein the at least one metalloproteinase inhibitor is a collagenase inhibitor.  
   
   
       48 . The composition of  claim 44  wherein the at least one metalloproteinase inhibitor is an aminocarboxylic acid or polyaminocarboxylic acid or a salt thereof.  
   
   
       49 . The composition of  claim 44  wherein the at least one metalloproteinase inhibitor is ethylenediaminetetraacetic acid or a salt thereof or is ethylenediaminetriacetic acid or a salt thereof.  
   
   
       50 . The composition of  claim 44  wherein the at least one metalloproteinase inhibitor is ethylenediaminetetraacetic acid, ethylenediaminetetraacetic acid disodium salt, ethylenediaminetetraacetic acid trisodium salt, ethylenediaminetetraacetic acid tetrasodium salt, ethylenediaminetetraacetic acid dipotassium salt, ethylenediaminetetraacetic acid tripotassium salt, ethylenediaminetetraacetic acid ammonium salt, ethylenediaminetetraacetic acid diammonium salt, or combinations thereof.  
   
   
       51 . The composition of  claim 44  wherein the at least one metalloproteinase inhibitor is ethylene glycol-bis(β-aminoethylether)-N,N,N′N′-tetraacetic acid or a salt thereof.  
   
   
       52 . The composition of  claim 44  wherein the at least one metalloproteinase inhibitor is ethylene glycol-bis(β-aminoethylether)-N,N,N′N′-tetraacetic acid tetrasodium salt.  
   
   
       53 . The method of  claim 44 , wherein the at least one metalloproteinase inhibitor is S,S′-ethylenediamine disuccinic acid, 1,2-diaminocyclohexene-N,N,N′,N′-tetraacetic acid, N-(2-hydroxyethyl)ethylenediamine-N,N′-triacetic acid, methylglycinediacetic acid, N,N-bis(carboxymethyl)glutamate, ortho-phenanthroline, 8-hydroxyquinoline, amino-tris methylene phosphonic acid, diethylene triamine pentamethylene phosphonic acid, 2-phosphono-1,2,4-butanetricarboxylic acid, hydroxyethylidene-diphosphonic acid, 2-methylpentanediamine tetrakis (methylene phosphonic acid) and 1,2, diaminocyclohexanetetrakis (methylene phosphonic acid), citric acid, a salt of citric acid, gluconic acid, a salt of gluconic acid, cysteine, iodoacetic acid or sodium iodoacetate or combinations thereof.  
   
   
       54 . The composition of  claim 44  wherein the at least one metalloproteinase inhibitor is present on the surface of the animal skin or hide in an amount of from about 0.00001% to about 10% by weight based on the weight of the animal skin or hide.  
   
   
       55 . A method of preventing or inhibiting putrefaction, degradation, and/or deterioration of an animal skin or hide, the method comprising applying at least one metalloproteinase inhibitor to the animal skin or hide, wherein the at least one metalloproteinase inhibitor is not admixed with a solid extender or carrier material.