Oncoprotein protein kinase

Abstract

An isolated polypeptide (JNK) characterized by having a molecular weight of 46 kD as determined by reducing SDS-PAGE, having serine and threonine kinase activity, phosphorylating the c-Jun N-terminal activation domain and polynucleotide sequences and method of detection of JNK are provided herein. JNK phosphorylates c-Jun N-terminal activation domain which affects gene expression from AP-1 sites.

1 . A method for identifying a compound which affects the activity of JNK Protein Kinase characterized by its ability to phosphorylate the c-Jun N-terminal activation domain comprising: 
 a) incubating the compound and the kinase or a polynucleotide encoding the kinase, wherein the incubation is carried out under conditions sufficient to allow the components to interact; and    b) measuring the effect of the compound on the kinase or polynucleotide encoding the kinase.    
     
     
         2 . A method for identifying a compound which affects the activity of a JNK protein kinase characterized by its ability to phosphorylate the c-Jun N-terminal activation domain comprising: 
 a) administering the compound to T cells expressing the kinase which are cultured under conditions sufficient to allow the components to interact; and    b) measuring the effect of the compound on the phosphorylated state of c-Jun.    
     
     
         3 . A method of  claim 2  whereby the compound is further assayed for its effect on the activated state of the T cells.  
     
     
         4 . The method of  claim 3  whereby the T cells are Jurkat cells.