Method and composition for resuscitation

Abstract

The key obstacle for current resuscitation is the acutely limited time window. The major reason for the limited time window is that the brain is extremely vulnerable to hypoxic-ischemic insult. The existence of cerebrospinal fluid (CSF) is the major reason why brain and spinal cord is so vulnerable. A method and a composition for resuscitation of cardiac arrest are provided. The method includes steps of inhibiting CSF production, removing CSF or replacing the CSF with invented composition plus conventional CPR. The composition includes colloidal osmotic agent, insulin, elevated magnesium concentration and ATP in artificial CSF.

1 . A method for resuscitating cardiac arrest in a mammal, comprising at least two steps of: 
 a. Administering a cerebrospinal fluid (CSF) production-suppressing agent in an amount effective to reduce or stop CSF production; wherein said CSF production-suppressing agent is Furosemide or Acetazolamide.    b. Removing the CSF from the subarachnoid space in central nervous system.    c. Conventional cardiopulmonary resuscitation (CPR) procedure including open airway, breathing, and circulation.    
     
     
         2 . A method for resuscitating cardiac arrest in a mammal, comprising at least two steps of: 
 a. Administering a cerebrospinal fluid (CSF) production-suppressing agent in an amount effective to reduce or stop CSF production; wherein said CSF production-suppressing agent is Furosemide or Acetazolamide.    b. Removing the CSF from the subarachnoid space in central nervous system and followed by injecting a volume of composition approximately equal or less to the volume of CSF withdrawn into said subarachnoid spaces; wherein said composition comprising of a mixture of at least one component selected from the group consisting of Albumin 0.1-20 gram/dl, gelatin 0.1-10 gram/dl, insulin 1 to 60 μU/ml and ATP 0.001 to 0.05 mM in an artificial CSF, wherein said artificial CSF comprising of Na 120-155 meq/L, K 0.1-5.0 meq/L, Ca 0.1-3.0 meq/L, P 0.1-10 meq/L, Cl 120-155 meq/L, Mg 0.9-8 meq/L, and water; wherein said composition has a Ph value between 6.8-7.0.    d. Conventional cardiopulmonary resuscitation (CPR) procedure including open airway, breathing, and circulation.    
     
     
         3 . A method for resuscitating cardiac arrest in a mammal, comprising at least two steps of: 
 a. Administering a cerebrospinal fluid (CSF) production-suppressing agent in an amount effective to reduce or stop CSF production; wherein said CSF production-suppressing agent is Furosemide or Acetazolamide.    b. Removing the CSF from the subarachnoid space in central nervous system and followed by injecting a volume of composition into one point of said subarachnoid space and draining out from other point of said subarachnoid spac; wherein said composition comprising of a mixture of at least one component selected from the group consisting of Albumin 0.1-20 gram/dl, gelatin 0.1-10 gram/dl, insulin 1 to 60 μU/ml and ATP 0.001 to 0.05 mM in an artificial CSF, wherein said artificial CSF comprising of Na 120-155 meq/L, K 0.1-5.0 meq/L, Ca 0.1-3.0 meq/L, P 0.1-10 meq/L, Cl 120-155 meq/L, Mg 0.9-8 meq/L, and water; wherein said composition has a Ph value between 6.8-7.0.    d. Conventional cardiopulmonary resuscitation (CPR) procedure including open airway, breathing, and circulation.    
     
     
         4 . A method for resuscitating cardiac arrest in a mammal according to step b in  claim 1 , wherein removing the CSF from the subarachnoid space in central nervous system is performed from at least one point selected from the lumber, cisterna magna, lateral ventricle and skull above each cerebral hemisphere.  
     
     
         5 . A method for resuscitating cardiac arrest in a mammal according  claim 4 , the patient is in a sit position when removing the CSF.  
     
     
         6 . A method for resuscitating cardiac arrest in a mammal according to step b in  claim 2 , wherein removing the CSF from the subarachnoid space and injecting said composition are performed from at least one point selected from the lumber, cisterna magna, lateral ventricle and skull above each cerebral hemisphere.  
     
     
         7 . A method for resuscitating cardiac arrest in a mammal according  claim 6 , the patient is in a sit position when removing the CSF.  
     
     
         8 . A method for resuscitating cardiac arrest in a mammal according to step b in  claim 3 , wherein removing the CSF, injecting and draining said composition is performed from at least one point selected from the lumber, cistema magna, lateral ventricle and skull above each cerebral hemisphere.  
     
     
         9 . A method for resuscitating cardiac arrest in a mammal according  claim 8 , the patient is in a sit position when removing the CSF.  
     
     
         10 . A method for resuscitating cardiac arrest in a mammal according  claim 6 , the ICP is maintained at 0-70 mm H 2 O.  
     
     
         11 . A method for resuscitating cardiac arrest in a mammal according  claim 8 , the ICP is maintained at 0-70 mm H 2 O.