Transposon and transposase genes of rice, transposase, transformant and method of use

Abstract

A transposon gene of rice has homology to SEQ. ID NO:3, and a transposase gene of rice has homology to bases 2959-4407 of SEQ. ID NO:3 or encodes SEQ. ID NO:5 or a variant thereof, and plasmids and transformants thereof. A transposase has SEQ. ID NO.:5 or a variant thereof. A method of transposing the transposon gene, of rice ( Oryza sativa ) having homology to SEQ. ID NO: 1 , comprises transducing a transposon gene of rice having homology to SEQ. ID NO:3, or transposase gene of rice having homology to SEQ. ID NO:4.

1 - 20 . (canceled)  
     
     
         21 . A transposon gene of rice, consisting essentially of a nucleotide sequence that is at least 90% homologous to SEQ ID NO: 3.  
     
     
         22 . A transposase gene of rice, consisting essentially of a nucleotide sequence that is at least 75% homologous to bases 2959-4407 of SEQ ID NO: 3.  
     
     
         23 . A transposase gene, encoding a protein consisting essentially of SEQ ID NO: 5 or SEQ ID NO: 5, wherein one or more amino acids are deleted, substituted or added.  
     
     
         24 . A transposase, consisting essentially of SEQ ID NO: 5 or SEQ ID NO: 5, wherein one or several amino acids are deleted, substituted or added.  
     
     
         25 . A plasmid, comprising the transposon gene of  claim 21 .  
     
     
         26 . A plasmid, comprising a promoter, and the transposase gene of  claim 22 .  
     
     
         27 . A plasmid, comprising a promoter, and the transposase gene of  claim 23 .  
     
     
         28 . A transformant, comprising a host carrying the transposon gene of  claim 21 , which is transduced.  
     
     
         29 . A transformant, comprising a host carrying a promoter and the transposase gene of  claim 22 , which are transduced.  
     
     
         30 . A transformant, comprising a host carrying a promoter and the transposase gene of  claim 23 , which are transduced.  
     
     
         31 . A method for transposing a nucleotide sequence which is at least 95% homologous to SEQ ID NO: 1, comprising transducing the transposon gene of  claim 21  to produce a transformant.  
     
     
         32 . The method of  claim 31 , further comprising subjecting the transformant to anther culture or treating it with a chemical agent.  
     
     
         33 . A method for transposing a nucleotide sequence which is at least 95% homologous to SEQ ID NO: 1, comprising transducing a promoter and the transposon gene of  claim 22  to produce a transformant.  
     
     
         34 . The method of  claim 33 , further comprising subjecting the transformant to anther culture or treating it with a chemical agent.  
     
     
         35 . A method for transposing a nucleotide sequence which is at least 95% homologous to SEQ ID NO: 1, comprising transducing a promoter and the transposon gene of  claim 23  to produce a transformant.  
     
     
         36 . The method of  claim 35 , further comprising subjecting the transformant to anther culture or treating it with a chemical agent.