Phenolic binding peptides

Abstract

The present application relates to peptides which bind to tannin, polyphenolic or anthocyanin compounds, and particularly to tea and wine stains on a fabric or other surface. The invention also concerns binding peptide conjugates which includes a binding peptide coupled to an agent and the use of the binding peptide conjugate for delivering an agent to a desired target.

1 . A binding peptide comprising an amino acid sequence shown in any one of SEQ ID NOs. 1-316 and a binding peptide having at least 70% sequence identity thereto.  
   
   
       2 . A binding peptide consisting essentially of an amino acid sequence shown in any one of SEQ ID NOs. 1-316 and a binding peptide having at least 70% sequence identity thereto.  
   
   
       3 . The binding peptide of  claim 1 , wherein the peptide is selected from the group consisting of KTPSPHG (SEQ ID NO. 1); PNTTRHS (SEQ ID NO. 2); LWTSPQL (SEQ ID NO. 8); TNNTSPT (SEQ ID NO. 24); SPTSTNS (SEQ ID NO. 43); TUTIPFA (SEQ ID NO. 77); SWNTSPL (SEQ ID NO. 80); QAVKASHATMYL (SEQ ID NO. 97); SYDLIPPRSGLA (SEQ ID NO. 104); DPNTTSH (SEQ ID NO. 118); KASHLVP (SEQ ID NO: 132); LPTSTLT (SEQ ID NO. 139); QNQKSTT (SEQ ID NO. 158); SIIPPRQ (SEQ ID NO. 168); WSNKPLSPNDLR (SEQ ID NO. 193) and peptides having at least 75% amino acid sequence identity thereto.  
   
   
       4 . The binding peptide of  claim 1 , having a repeatable motif selected from the group consisting of 
 LPL (SEQ ID NOs. 120, 123, 115 and 250);    FAT (SEQ ID NOs. 125, 227 and 235);    STT (SEQ ID NOs. 90, 158, 230 and 310);    HSP (SEQ ID NOs. 18, 252 and 307);    TNK (SEQ ID NOs. 40, 259 and 287);    SPL (SEQ ID NOs. 53, 80, 152, 229, 232 and 292);    THS (SEQ ID NOs. 62, 209 and 290);    TSP (SEQ ID NOs. 8, 24, 80, 223 and 291);    SPT (SEQ ID NOs. 24, 43 and 266);    AQT (SEQ ID NOs. 59, 134 and 205);    NSS (SEQ ID NOs. 31, 86, 213, 227 and 278);    PAL (SEQ ID NOs. 109, 224 and 256);    SGL (SEQ ID NOs. 104, 284 and 298); and    TQT (SEQ ID NOs. 105, 281 and 287) and a binding peptide having the repeatable motif and at least 75% amino acid sequence identity to a binding peptide having the repeatable motif and listed herein.    
   
   
       5 . The binding peptide of  claim 1 , wherein said peptide binds to a compound selected from the group consisting of tannin, anthocyanin and phenolic compounds.  
   
   
       6 . The binding peptide of  claim 1 , wherein said peptide binds to a tea or wine stain.  
   
   
       7 . The binding peptide of  claim 6 , wherein the peptide binds to a tea or wine stain on a fabric.  
   
   
       8 . The binding peptide of  claim 6 , wherein the peptide binds to a tea or wine stain on a surface.  
   
   
       9 . The binding peptide of  claim 8 , wherein the surface is selected from the group consisting of ceramic, glass, wood, paper, skin, hair and plastic.  
   
   
       10 . The binding peptide of  claim 1 , further comprising a cysteine amino acid residue at the N and C terminus of said peptide.  
   
   
       11 . A binding peptide conjugate comprising a binding peptide of  claim 1 , linked to an agent.  
   
   
       12 . A binding peptide conjugate comprising a binding peptide of  claim 10 , linked to an agent.  
   
   
       13 . The conjugate according to  claim 11 , wherein said agent is a protein.  
   
   
       14 . The conjugate of  claim 13 , wherein the protein is an enzyme.  
   
   
       15 . The conjugate of  claim 14 , wherein said enzyme is an enzyme that catalyzes an oxidation-reduction reaction and is selected from the group consisting of laccases, phenol oxidases, catalases, bilirubrin oxidases, glucose oxidases, and peroxidases.  
   
   
       16 . The conjugate of  claim 12 , wherein said binding peptide is covalently linked to said agent.  
   
   
       17 . The conjugate of  claim 12 , wherein said binding peptide and said agent are separated by a linker.  
   
   
       18 . An enzymatic composition comprising a binding peptide of  claim 1 , an enzyme, and one or more surfactants.  
   
   
       19 . The enzymatic composition of  claim 18 , wherein said enzymatic composition is a detergent composition.  
   
   
       20 . An enzymatic composition comprising a) a binding peptide conjugate which comprises a binding peptide of  claim 1  linked to an agent, wherein the agent is an enzyme and b) one or more surfactants.  
   
   
       21 . A method for modifying a tea or wine stain on a fabric or a surface comprising contacting a fabric or a surface having a tea or wine stain thereon with the enzymatic composition of  claim 18 .  
   
   
       22 . The method of  claim 21 , wherein the surface is a ceramic surface.  
   
   
       23 . The method of  claim 21 , wherein the surface is skin or hair.  
   
   
       24 . The method of  claim 21 , wherein the modification is removing the tea or wine stain.  
   
   
       25 . The method of  claim 21 , wherein the modification is enhancing the tea or wine stain.  
   
   
       26 . A method for delivering an agent to a target comprising a) conjugating an agent with a binding peptide of  claim 1  to form a binding peptide conjugate and b) exposing a target to the binding peptide conjugated, wherein the binding peptide conjugate binds to said target.  
   
   
       27 . The method according to  claim 26 , wherein the target is a tea or wine stain.  
   
   
       28 . The method according to  claim 26 , wherein the agent is an enzyme  
   
   
       29 . A polynucleotide sequence encoding a binding peptide of  claim 1 .  
   
   
       30 . A polynucleotide sequence encoding a binding peptide conjugate of  claim 11 .  
   
   
       31 . A polynucleotide sequence encoding a binding peptide of  claim 10 .  
   
   
       32 . An expression vector comprising a polynucleotide sequence encoding a binding peptide of  claim 1  which is operably linked to a promoter and termination sequence.  
   
   
       33 . An expression vector comprising a polynucleotide sequence encoding a binding peptide conjugate of  claim 11  which is operably linked to a promoter and termination sequence.  
   
   
       34 . A host cell comprising the expression vector of  claim 32 .  
   
   
       35 . A host cell comprising the expression vector of  claim 33.