Mammalian neuralized family transcriptional regulators and uses

Abstract

The disclosure relates to isolated polynucleotides and purified polypeptides of the Neu family of proteins, which have been shown to demonstrate transcriptional regulatory activity. For example, the purified polynucleotide can encode a Neu polypeptide, wherein the Neu polypeptide comprises at least one neuralized homology repeat domain and a C3HC4 RING-zinc finger domain is disclosed. A purified Neu polypeptide, wherein the Neu polypeptide comprises at least one neuralized homology repeat domain and a C3HC4 RING-zinc finger domain is disclosed. Antibodies capable of specifically binding to the disclosed Neu polypeptides are disclosed. Vectors expressing the disclosed Neu protein coding regions and host cells containing the vectors are disclosed. Methods of making the Neu proteins disclosed are also provided, as are method of identifying binding partners that interact with a Neu protein family member.

1 - 30 . (canceled)  
     
     
         31 . A purified neuralized (Neu) polypeptide, comprising at least one neuralized homology repeat domain and a C3HC4 RING-zinc finger domain, wherein said Neu polypeptide has at least 90% sequence identity to SEQ ID NO:22 and is capable of suppressing transcription.  
     
     
         32 . The purified Neu polypeptide according to  claim 31 , wherein said Neu polypeptide has at least 95% sequence identify to SEQ ID NO:22.  
     
     
         33 . The purified Neu polypeptide according to  claim 31 , wherein said Neu polypeptide comprises SEQ ID NO:22.  
     
     
         34 . The purified Neu polypeptide according to  claim 31 , wherein said neuralized homology repeat domain comprises SEQ ID NO:48.  
     
     
         35 . An antibody capable of specifically binding to an isolated Neu polypeptide according to  claim 31 .  
     
     
         36 . The antibody according to  claim 35 , wherein said antibody specifically binds to a polypeptide comprising at least 10 consecutive amino acids of said Neu polypeptide.  
     
     
         37 . The antibody according to  claim 35 , wherein said antibody is a monoclonal antibody.