Nucleic acid isolation unit and method using intercalator

Abstract

Provided are nucleic acid isolation unit and method. The method includes immobilizing an aromatic compound-containing nucleic acid intercalator on a solid support; contacting a first buffer solution containing a nucleic acid sample to be purified to the intercalator immobilized on the solid support to bind the intercalator with nucleic acids contained in the nucleic acid sample; cleaning the resultant structure where the nucleic acids are bound to the intercalator immobilized on the solid support; and eluting the nucleic acids with a second buffer solution.

1 . A nucleic acid isolation unit comprising: 
 a solid support;    a polymer layer coated on the solid support; and    a nucleic acid intercalator comprising an aromatic compound immobilized on the polymer layer.    
     
     
         2 . The nucleic acid isolation unit of  claim 1 , wherein the solid support is in the form of a plate or a bead.  
     
     
         3 . The nucleic acid isolation unit of  claim 1 , wherein the polymer layer comprises at least one functional group selected from the group consisting of a hydroxy group, an amino group, a thiol group, a carboxy group, an alkoxy group, and a formyl group.  
     
     
         4 . The nucleic acid isolation unit of  claim 1 , wherein the polymer layer comprises at least one polymer selected from the group consisting of polysilane, polyalcohol, polyvinyl, and polystyrene.  
     
     
         5 . The nucleic acid isolation unit of  claim 1 , wherein the nucleic acid intercalator is covalently bound to the polymer layer.  
     
     
         6 . The nucleic acid isolation unit of  claim 1 , wherein the nucleic acid intercalator is a substituted or unsubstituted aromatic compound of 10 to 100 carbon atoms.  
     
     
         7 . The nucleic acid isolation unit of  claim 6 , wherein the aromatic compound has 2 to 6 benzene rings and the benzene rings are attached to each other as a pendant group or are partially or wholly fused.  
     
     
         8 . The nucleic acid isolation unit of  claim 6 , wherein the aromatic compound is at least one selected from the group consisting of naphthalene, anthracene, phenanthrene, pyrene, chrysene, and tetracene.  
     
     
         9 . A nucleic acid isolation method using an intercalator comprising: 
 immobilizing an aromatic compound-containing nucleic acid intercalator on a solid support;    contacting a first buffer solution containing a nucleic acid sample to be purified to the intercalator immobilized on the solid support to bind the intercalator with nucleic acids contained in the nucleic acid sample;    cleaning the resultant structure where the nucleic acids are bound to the intercalator immobilized on the solid support; and    eluting the nucleic acids with a second buffer solution.    
     
     
         10 . The nucleic acid isolation method of  claim 9 , wherein the nucleic acid intercalator is a substituted or unsubstituted aromatic compound of 10 to 100 carbon atoms.  
     
     
         11 . The nucleic acid isolation method of  claim 10 , wherein the aromatic compound has 2 to 6 benzene rings and the benzene rings are attached to each other as a pendant group or are partially or wholly fused.  
     
     
         12 . The nucleic acid isolation method of  claim 10 , wherein the aromatic compound is at least one selected from the group consisting of naphthalene, anthracene, phenanthrene, pyrene, chrysene, and tetracene.  
     
     
         13 . The nucleic acid isolation method of  claim 9 , wherein the nucleic acids are double-stranded DNAs or single-stranded DNAs.  
     
     
         14 . The nucleic acid isolation method of  claim 9 , wherein the first buffer solution has a salt concentration of 0.1 to 0.3M.  
     
     
         15 . The nucleic acid isolation method of  claim 9 , wherein the second buffer solution has a salt concentration of 0.5 to 2M.  
     
     
         16 . The nucleic acid isolation method of  claim 9 , wherein the second buffer solution is a nucleic acid amplification buffer.  
     
     
         17 . The nucleic acid isolation method of  claim 16 , wherein the nucleic acid amplification buffer is a PCR (Polymerase Chain Reaction) buffer.  
     
     
         18 . The nucleic acid isolation method of  claim 9 , wherein the second buffer solution has a temperature of 70 to 100° C.