Visualization of avian eggs

Abstract

The invention includes methods of visualizing and manipulating avian eggs employing TPLSM.

1 . A method comprising: 
 visualizing a nucleus of an avian egg by TPLSM.    
   
   
       2 . The method of  claim 1  wherein a substance is injected into the nucleus.  
   
   
       3 . The method of  claim 1  wherein the nucleus is injected with nucleic acid.  
   
   
       4 . The method of  claim 1  wherein the egg is an oocyte.  
   
   
       5 . The method of  claim 1  wherein the avian egg is an egg of an avian selected from the group consisting of avians of chicken, turkey, duck, goose, quail, pheasant, parrot, finche, hawk, crow, ratite, ostrich, emu and cassowary.  
   
   
       6 . A method comprising: 
 visualizing a nucleus of an avian embryo by TPLSM.    
   
   
       7 . The method of  claim 6  wherein the embryo comprises more than one cell.  
   
   
       8 . The method of  claim 6  wherein the embryo contains between 1 and 100,000 cells.  
   
   
       9 . The method of  claim 6  wherein a substance is injected into the nucleus.  
   
   
       10 . The method of  claim 6  wherein the nucleus is injected with nucleic acid.  
   
   
       11 . The method of  claim 10  wherein the nucleic acid comprises a transgene.  
   
   
       12 . The method of  claim 6  wherein the avian egg is an egg of an avian selected from the group consisting of avians of chicken, turkey, duck, goose, quail, pheasant, parrot, finche, hawk, crow, ratite, ostrich, emu and cassowary.  
   
   
       13 . The method of  claim 6  wherein the egg is an early stage embryo.  
   
   
       14 . The method of  claim 6  wherein the egg is selected from the group consisting of a stage I, stage II, stage III, stage IV, stage V, stage VI, stage VII, stage VIII, stage IX, stage X, stage XI and stage XII embryo.  
   
   
       15 . A method comprising visualizing an avian egg using TPLSM and injecting an artificial chromosome into a nucleus.  
   
   
       16 . The method of  claim 15  wherein the egg is an oocyte.  
   
   
       17 . The method of  claim 15  wherein the egg is a zygote.  
   
   
       18 . The method of  claim 15  wherein the avian egg is an egg of an avian selected from the group consisting of avians of chicken, turkey, duck, goose, quail, pheasant, parrot, finche, hawk, crow, ratite, ostrich, emu and cassowary.  
   
   
       19 . The method of  claim 15  wherein the embryo is an early stage embryo.  
   
   
       20 . The method of  claim 15  wherein the embryo is selected from the group consisting of a stage I, stage II, stage III, stage IV, stage V, stage VI, stage VII, stage VIII, stage IX, stage X, stage XI and stage XII embryo.