Method for identifying active anti-apoptosis compounds

Abstract

The invention relates to a method for identifying substances with antiapoptotic activity, where i) cells which express both CD47 and the integrin α v β 3 are cultured, ii) the cells are caused to produce an apoptosis-inducing substance, and/or a substance or substances which induces/induce apoptosis is/are added, iii) the test substance is added, and iv) the apoptosis rate is measured, and to the substances which can be identified with the method.

1 . A method for identifying substances with antiapoptotic activity, comprising 
 i) culturing cells which express both CD47 and the integrin α v β 3 ,    ii) causing the cells to produce an apoptosis-inducing substance, and/or adding a substance which simultaneously binds CD47 and α v β 3  by culturing under static or turbulent flow conditions, thereby inducing apoptosis,    iii) adding a test substance, and    iv) determining a change in apoptosis rate by determining the portion of cells with apoptotic nuclei,    wherein a decrease in apoptotic rate in step iv) is indicative of the test substance having antiapoptotic activity.