Nucleotide sequences which code for the dep34 gene

Abstract

The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the dep34 gene, and a host-vector system having a coryneform host bacterium in which the dep34 gene is present in attenuated form and a vector which carries at least the dep34 gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

1 - 25 . (canceled)  
     
     
         26 . A process for the fermentative preparation of L-amino acids in  Corynebacterium glutamicum  bacteria, comprising: 
 a) fermenting the bacteria, in which at least the dep34 gene is eliminated in a medium and for a time suitable for the formation of the L-amino acids, wherein prior to said elimination said bacteria contain the dep34 gene which comprises a nucleotide sequence which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 2.    
     
     
         27 . The method according to  claim 26 , further comprising: 
 b) isolating the L-amino acids.    
     
     
         28 . The method as claimed in  claim 26 , wherein the L-amino acid produced is L-lysine.  
     
     
         29 . The method as claimed in  claim 26 , wherein the bacteria comprise, at the same time, one or more endogenous  Corynebacterium glutamicum  genes which are overexpressed, wherein the one or more genes is/are selected from the group consisting of: 
 the gene which encodes dihydrodipicolinate synthase,    the gene which encodes glyceraldehyde 3-phosphate dehydrogenase,    the gene which encodes triose phosphate isomerase,    the gene which encodes 3-phosphoglycerate kinase,    the gene which encodes glucose 6-phosphate dehydrogenase,    the gene which encodes pyruvate carboxylase,    the gene which encodes malate-quinone oxidoreductase,    the gene which encodes an aspartate kinase,    the gene which encodes a protein which exports lysine,    the gene which encodes homoserine dehydrogenase,    the gene which encodes threonine dehydratase,    the gene which encodes acetohydroxy-acid synthase,    the gene which encodes dihydroxy-acid dehydratase, and    the gene which encodes for the Zwa1 protein.    
     
     
         30 . The method as claimed in  claim 26 , wherein the bacteria comprise, at the same time, one or more endogenous  Corynebacterium glutamicum  genes which are eliminated, wherein said one or more genes is/are selected from the group consisting of: 
 the gene which encodes phosphoenol pyruvate carboxykinase,    the gene which encodes glucose 6-phosphate isomerase,    the gene which encodes pyruvate oxidase, and    the gene which encodes the Zwa2 protein.    
     
     
         31 . The method as claimed in  claim 26 , wherein said polynucleotide comprises the nucleotides 259 to 1905 of SEQ ID NO: 1.  
     
     
         32 . The method as claimed in  claim 26 , wherein said polynucleotide comprises the nucleotides sequence of SEQ ID NO: 1.  
     
     
         33 . The method as claimed in  claim 26 , wherein said elimination is achieved by one of more methods of mutagenesis selected from the group consisting of deletion mutagenesis with deletion of two or more codons in the dep34 gene; insertion mutagenesis due to homologous mutagenesis recombination; and transition or transversion mutagenesis with the incorporation of a non-sense mutation in the dep34 gene.