Protein forming complex with c-fos protein, nucleic acid encoding the same and method of using the same

Abstract

Proteins that interact with c-Fos, nucleic acids encoding them and inhibitors utilizing them as well as methods for detecting an interaction and screening methods utilizing a protein that interacts with c-Fos are provided. Comprehensive analysis of transcription control factor complexes in a mouse brain cDNA library with c-Fos as a bait by using the cotranslation selection and screening of in vitro virus (IVV) and the C-terminal labeling method are conducted, thereby to analyze proteins unknown so far, proteins known so far, but unknown to form a complex with the c-Fos protein, and so forth.

1 . A protein of the following (a) or (b): 
 (a) a protein comprising any one of the amino acid sequences of SEQ ID NOS: 1 to 14, SEQ ID NOS: 15 to 19, SEQ ID NOS: 20 to 22, SEQ ID NOS: 47 to 56, SEQ ID NOS: 57 to 76, SEQ ID NOS: 77 to 81, SEQ ID NOS: 82 to 84, SEQ ID NOS: 85 to 86, SEQ ID NOS: 87 to 89, SEQ ID NOS: 90 to 91, SEQ ID NOS: 92 to 93, SEQ ID NOS: 94 to 95, SEQ ID NOS: 96 to 97, SEQ ID NOS: 98 to 99, SEQ ID NOS: 100 to 101, SEQ ID NO: 102 and SEQ ID NO: 103,    (b) a protein that comprises any one of the amino acid sequences of SEQ ID NOS:1 to 14, SEQ ID NOS: 15 to 19, SEQ ID NOS: 20 to 22, SEQ ID NOS: 47 to 56, SEQ ID NOS: 57 to 76, SEQ ID NOS: 77 to 81, SEQ ID NOS: 82 to 84, SEQ ID NOS: 85 to 86, SEQ ID NOS: 87 to 89, SEQ ID NOS: 90 to 91, SEQ ID NOS: 92 to 93, SEQ ID NOS: 94 to 95, SEQ ID NOS: 96 to 97, SEQ ID NOS: 98 to 99, SEQ ID NOS: 100 to 101, SEQ ID NO: 102 and SEQ ID NO: 103, including deletion, substitution or addition of one or several amino acid residues and interacts with a c-Fos protein.    
     
     
         2 . A protein according to  claim 1 , which comprises any one of the amino acid sequences of SEQ ID NOS: 1 to 14, SEQ ID NOS: 15 to 19, SEQ ID NOS: 20 to 22, SEQ ID NOS: 47 to 56, SEQ ID NOS: 57 to 76, SEQ ID NOS: 77 to 81, SEQ ID NOS: 82 to 84, SEQ ID NOS: 85 to 86, SEQ ID NOS: 87 to 89, SEQ ID NOS: 90 to 91, SEQ ID NOS: 92 to 93, SEQ ID NOS: 94 to 95, SEQ ID NOS: 96 to 97, SEQ ID NOS: 98 to 99, SEQ ID NOS: 100 to 101, SEQ ID NO: 102 and SEQ ID NO: 103.  
     
     
         3 . A nucleic acid encoding the protein according to  claim 1 .  
     
     
         4 . A nucleic acid of the following (a) or (b): 
 (a) a nucleic acid comprising any one of the nucleotide sequences of SEQ ID NOS: 23 to 38, SEQ ID NOS: 39 to 43, SEQ ID NOS: 44 to 46, SEQ ID NOS: 104 to 118, SEQ ID NOS: 119 to 140, SEQ ID NOS: 141 to 145, SEQ ID NOS: 146 to 148, SEQ ID NOS: 149 to 150, SEQ ID NOS: 151 to 153, SEQ ID NOS: 154 to 155, SEQ ID NOS: 156 to 157, SEQ ID NOS: 158 to 159, SEQ ID NOS: 160 to 161, SEQ ID NOS: 162 to 163, SEQ ID NOS: 164 to 165, SEQ ID NO: 166, and SEQ ID NO: 167,    (b) a nucleic acid that hybridizes with a nucleic acid comprising any one of the nucleotide sequences of SEQ ID NOS: 23 to 38, SEQ ID NOS: 39 to 43, SEQ ID NOS: 44 to 46, SEQ ID NOS: 104 to 118, SEQ ID NOS: 119 to 140, SEQ ID NOS: 141 to 145, SEQ ID NOS: 146 to 148, SEQ ID NOS: 149 to 150, SEQ ID NOS: 151 to 153, SEQ ID NOS: 154 to 155, SEQ ID NOS: 156 to 157, SEQ ID NOS: 158 to 159, SEQ ID NOS: 160 to 161, SEQ ID NOS: 162 to 163, SEQ ID NOS: 164 to 165, SEQ ID NO: 166, and SEQ ID NO: 167, under a stringent condition and encodes a protein that interacts with a c-Fos protein.    
     
     
         5 . A nucleic acid according to  claim 4 , which comprises any one of the nucleotide sequences of SEQ ID NOS: 23 to 38, SEQ ID NOS: 39 to 43, SEQ ID NOS: 44 to 46, SEQ ID NOS: 104 to 118, SEQ ID NOS: 119 to 140, SEQ ID NOS: 141 to 145, SEQ ID NOS: 146 to 148, SEQ ID NOS: 149 to 150, SEQ ID NOS: 151 to 153, SEQ ID NOS: 154 to 155, SEQ ID NOS: 156 to 157, SEQ ID NOS: 158 to 159, SEQ ID NOS: 160 to 161, SEQ ID NOS: 162 to 163, SEQ ID NOS: 164 to 165, SEQ ID NO: 166, and SEQ ID NO: 167.  
     
     
         6 . An inhibitor for an interaction between a protein that interacts with a c-Fos protein and the c-Fos protein, which comprises the protein according to  claim 1  or  2  or a protein translated from the nucleic acid according to any one of  claims 3  to  5  or  133  as an active ingredient.  
     
     
         7 . A method for detecting an interaction between a bait and a prey, which comprises bringing the bait and the prey into contact and detecting a complex formed by the contact, wherein the bait is the protein according to  claim 1  or  2  or a protein translated from the nucleic acid according to any one of  claims 3  to  5  or  133 .  
     
     
         8 . A method for screening for a prey that interacts with a bait, which comprises the step of detecting an interaction between the bait and a prey by the method according to  claim 7  and the step of selecting a prey for which an interaction was detected.  
     
     
         9 - 132 . (canceled)  
     
     
         133 . A nucleic acid encoding the protein according to  claim 2.