Neuropeptide Y receptor Y5 and nucleic acid sequences

Abstract

The present invention provides novel NPY/PYY receptor proteins and the nucleic acid sequence encoding them. The invention is directed to the isolation, characterization, and pharmacological use of these receptors and nucleic acids. In particular, this invention provides human and rat NPY/PYY receptors (which we call the NPY Y5 receptor) and nucleic acids. Also provided are recombinant expression constructs useful for transfecting cells and expressing the protein in vitro and in vivo. The invention further provides methods for detecting expression levels of the protein as well as methods for screening for receptor antagonists and agonists to be used for the treatment of obesity or anorexia, respectively.

1 - 34 . (canceled)  
     
     
         35 . A method of identifying a neuropeptide Y agonist or antagonist comprising contacting a potential agonist or antagonist molecule with a membrane or membrane preparation comprising a membrane or portion thereof of a cell expressing a nucleic acid having a nucleotide sequence substantially the same as SEQ ID NO: 1, SEO ID NO: 3, or SEQ ID NO: 5.  
     
     
         36 . A method of identifying a neuropeptide Y agonist or antagonist comprising contacting a potential agonist or antagonist molecule with a membrane or membrane preparation comprising a membrane or Portion thereof of a cell expressing a nucleic acid having a nucleotide sequence substantially the same as SEQ ID NO: 1, SEQ ID NO: 3, or SEQ ID NO: 5 wherein the cell is a mammalian cell.  
     
     
         37 . A neuropeptide Y antagonist comprising a compound identified according to  claim 36 .  
     
     
         38 . A method of suppressing the appetite of a mammal comprising administering to the mammal an appetite suppressing amount of a neuropeptide Y antagonist according to  claim 37 .  
     
     
         39 . A method of suppressing the appetite of a mammal according to  claim 38  wherein the amount of antagonist is from about 0.01 to about 100 mg/kg.  
     
     
         40 . A pharmaceutical composition comprising an effective appetite suppressing amount of an antagonist according to  claim 37  together with a pharmaceutically acceptable career.  
     
     
         41 . A neuropeptide Y agonist comprising a compound identified according to  claim 36 .  
     
     
         42 . A method of stimulating the appetite of a mammal comprising administering to the mammal an appetite stimulating amount of a neuropeptide Y agonist according to  claim 41 .  
     
     
         43 . A method of stimulating the appetite of a mammal according to  claim 42  wherein the amount of agonist is from about 0.01 to about 100 mg/kg.  
     
     
         44 . A pharmaceutical composition comprising an effective appetite stimulating amount of an agonist according to  claim 41  together with a pharmaceutically acceptable cartier.  
     
     
         45 . (canceled)