Nucleic acid encoding Schwannomin-Binding-Proteins and products related thereto

Abstract

In accordance with the present invention, there are provided novel Schwannomin-Binding-Proteins (SBPs). Nucleic acid sequences encoding such proteins and assays employing same are also disclosed. The invention SBPs can be employed in a variety of ways, for example, for the production of anti-SBP antibodies thereto, in therapeutic compositions and methods employing such proteins and/or antibodies. Also provided are transgenic non-human mammals that express the invention protein.

1 . Isolated nucleic acid encoding a Schwannomin-Binding-Protein (SBP), or a functional fragment thereof.  
     
     
         2 . Isolated nucleic acid encoding Schwannomin-Binding-Protein (SBP), or functional fragments thereof, selected from: 
 (a) DNA encoding the amino acid sequence set forth in SEQ ID Nos:2, 4, 6, 8 or 10, or    (b) DNA that hybridizes to the DNA of (a) under moderately stringent conditions, wherein said DNA encodes biologically active SBP, or    (c) DNA degenerate with respect to either (a) or (b) above, wherein said DNA encodes biologically active SBP.    
     
     
         3 . A nucleic acid according to  claim 2 , wherein said nucleic acid hybridizes under high stringency conditions to the SBP coding portion of any of SEQ ID NOs:1, 3, 5, 7 and 9.  
     
     
         4 . A nucleic acid according to  claim 2 , wherein the nucleotide sequence of said nucleic acid is substantially the same as set forth in any of SEQ ID NO:1, 3, 5, 7 and 9.  
     
     
         5 . A nucleic acid according to  claim 2 , wherein the nucleotide sequence of said nucleic acid is the same as that set forth in any of SEQ ID NOs:1, 3, 5, 7 and 9.  
     
     
         6 . A nucleic acid according to  claim 2 , wherein said nucleic acid is cDNA.  
     
     
         7 . A vector containing the nucleic acid of  claim 2 .  
     
     
         8 . Recombinant cells containing the nucleic acid of  claim 2 .  
     
     
         9 . An oligonucleotide comprising at least 15 nucleotides capable of specifically hybridizing with a the nucleotide sequence set forth in any of SEQ ID NOs:1, 3, 5, 7 and 9.  
     
     
         10 . An oligonucleotide according to  claim 9 , wherein said oligonucleotide is labeled with a detectable marker.  
     
     
         11 . An antisense-nucleic acid capable of specifically binding to mRNA encoded by said nucleic acid according to  claim 2 .  
     
     
         12 . A kit for detecting the presence of the SBP cDNA sequence comprising at least one oligonucleotide according to  claim 10 .  
     
     
         13 . An isolated Schwannomin-Binding-Protein (SBP) characterized by having ability to bind to schwannomin.  
     
     
         14 . A SBP according to  claim 13 , wherein the amino acid sequence of said protein comprises substantially the same sequence as any of SEQ ID Nos:2, 4, 6, 8 or 10.  
     
     
         15 . A SBP according to  claim 14  comprising the same amino acid sequence as set forth in any of SEQ ID Nos:2, 4, 6, 8 or 10.  
     
     
         16 . A SBP according to  claim 13 , wherein said protein is encoded by a nucleotide sequence comprising substantially the same nucleotide sequence as set forth in SEQ ID Nos:1, 3, 5, 7 or 9.  
     
     
         17 . A SBP according to  claim 16 , wherein said protein is encoded by a nucleotide sequence comprising the same sequence as set forth in SEQ ID Nos:1, 3, 5, 7 or 9.  
     
     
         18 . A method for expression of a SBP-related protein, said method comprising culturing cells of  claim 8  under conditions suitable for expression of said SBP.  
     
     
         19 . An isolated anti-SBP antibody having specific reactivity with a SBP according to  claim 13 .  
     
     
         20 . Antibody according to  claim 19 , wherein said antibody is a monoclonal antibody.  
     
     
         21 . An antibody according to  claim 20 , wherein said antibody is a polyclonal antibody.  
     
     
         22 . A composition comprising an amount of the antisense-nucleic acid according to  claim 11  effective to inhibit expression of a human SBP and an acceptable hydrophobic carrier capable of passing through a cell membrane.  
     
     
         23 . A transgenic nonhuman mammal expressing exogenous nucleic acid encoding a SBP.  
     
     
         24 . A transgenic nonhuman mammal according to  claim 23 , wherein said nucleic acid encoding said SBP has been mutated, and wherein the SBP so expressed is not native SBP.  
     
     
         25 . A transgenic nonhuman mammal according to  claim 23 , wherein the transgenic nonhuman mammal is a mouse.  
     
     
         26 . A method for identifying nucleic acids encoding a mammalian SBP, said method comprising: 
 contacting a sample containing nucleic acids with an oligonucleotide according to  claim 9 , wherein said contacting is effected under high stringency hybridization conditions, and identifying compounds which hybridize thereto.    
     
     
         27 . A method for detecting the presence of a human SBP in a sample, said method comprising contacting a test sample with an antibody according to  claim 19 , detecting the presence of an antibody-SBP complex, and therefor detecting the presence of a human SBP in said test sample.  
     
     
         28 . Single strand DNA primers for amplification of SBP nucleic acid, wherein said primers comprise a nucleic acid sequence derived from the nucleic acid sequences set forth as SEQ ID NOs:1, 3, 5, 7 and 9.  
     
     
         29 . A method for modulating the activity of an oncogenic protein, comprising contacting said oncogenic proteins with a substantially pure SBP, or a oncogenic protein-binding fragment thereof.