Bioanalysis systems including optical integrated circuit

Abstract

Optical Integrated Circuits (OIC) in Surface Plasmon Resonance (SPR) Analysis Systems combined with micorarray or microwell plates to provide enhanced sensitivity, stability, speed of analysis and reduced size are disclosed. Using the OIC with other optical analysis methods to provide enhance analysis systems is also disclosed.

1 .- 23 . (canceled)  
   
   
       24 . A bioanalysis system for monitoring binding interactions between members of a binding pair, comprising: 
 an illumination source operable to illuminate at least one incident region of a support comprising a metal film;    a collector operable to collect illumination reflected from the metal film;    wherein at least one of the illumination source and the collector comprise an optical integrated circuit, the optical integrated circuit comprises at least one arrayed waveguide grating spectrometer.    
   
   
       25 . The bioanalysis system according to  claim 24 , wherein the support comprising a metal film further comprises a first medium adjacent a first side of the metal film and a second medium adjacent a second side of the metal film, and at least one assay region on the second side of the metal film opposing the incident region.  
   
   
       26 . The bioanalysis system according to  claim 24 , wherein the arrayed waveguide grating spectrometer comprises more than about 500 channels.  
   
   
       27 . The bioanalysis system according to  claim 25 , wherein the second medium comprises a first member of a binding pair and a second member of a binding pair.  
   
   
       28 . The bioanalysis system according to  claim 24 , wherein the metal film comprises at least about 100 incident regions and at least about 100 corresponding assay regions, the illumination source operable to illuminate the at least about 100 incident regions.  
   
   
       29 . The bioanalysis system according to  claim 24 , wherein the metal film is comprised within one of a gene chip, a DNA chip, or a protein chip.  
   
   
       30 . The bioanalysis system according to  claim 27 , wherein at least one of the first member of a binding pair and the second member of a binding pair have a molecular weight of about 2,000 or less.  
   
   
       31 . The bioanalysis system according to  claim 24 , wherein the metal film comprises at least one of gold, silver, aluminum, and molybdenum, and the first medium comprises at least one of a plastic, borosilicate glass, phosphosilicate glass, and silica glass.  
   
   
       32 . The bioanalysis system according to  claim 24 , wherein the optical integrated circuit further comprises an integrated optical switch.  
   
   
       33 . A method of monitoring binding interactions between members of a binding pair, comprising: 
 illuminating a metal film, a first member of a binding pair immobilized on a side of the metal film;    contacting the first member of a binding pair with a second member of a binding pair;    collecting illumination reflected from the metal film; and    analyzing properties of the collected illumination; wherein at least one of illuminating the metal film and collecting illumination comprises using an optical integrated circuit comprising at least one arrayed waveguide grating spectrometer.    
   
   
       34 . The method according to  claim 33 , wherein illuminating the metal film comprises illuminating the metal film through a first medium adjacent one side of the metal film, an assay medium comprising the first member of a binding pair being immobilized on a second side of the metal film.  
   
   
       35 . The method according to  claim 33 , wherein illuminating the metal film comprises using light having a wavelength from about 390 nm to about 2,500 nm.  
   
   
       36 . The method according to  claim 34 , wherein at least about 225 assay media each comprising a first member of a binding pair are immobilized on the second side of the metal film.  
   
   
       37 . The method according to  claim 33 , wherein analyzing properties of the reflected light determines at least one of a) measuring an amount of the second member of a binding pair bound to the first member of a binding pair, b) whether or not binding occurs between the first member of a binding pair and the second member of a binding pair, and c) distinguishing between specific and non-specific binding between the first member of a binding pair and the second member of a binding pair.  
   
   
       38 . The method according to  claim 33 , wherein the optical integrated circuit uses a 2×N optical switch to directing light at the metal film.  
   
   
       39 . The method according to  claim 36 , wherein the assay media each have a volume of about 0.5 μL or less.  
   
   
       40 . The method according to  claim 33 , wherein at least one of the first member of a binding pair and the second member of a binding pair have a molecular weight of about 2,000 or less.  
   
   
       41 . The method according to  claim 33 , wherein the first member of a binding pair and the second member of a binding pair are independently selected from the group consisting of antigen, antibody, hormone, hormone receptor, polynucleotide, avidin, streptavidin, biotin, enzyme, enzyme substrate or inhibitor, lectins, specific carboxyhydrate, lipids, lipid binding proteins or membrane associated proteins, polynucleotides, polynucleotide binding proteins, receptor, transmitter, drug, target, protein, small molecule having a molecular weight of about 2,000 or less, polynucleic acid, DNA, and RNA.  
   
   
       42 . The method according to  claim 33 , wherein the optical integrated circuit further comprises an integrated optical switch.  
   
   
       43 . The method according to  claim 33 , wherein the metal film is comprised within a disposable protein chip array.