Device and method for multiple analyte detection

Abstract

The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device can comprise wells and each well can comprise an analyte-specific reagent effective to react with a selected analyte that may be present in the sample. Also disclosed are methods utilizing the device.

1 . A device comprising: 
 a substrate comprising two or more layers and a plurality of wells formed therein, wherein at least one of the layers comprises a coated metal layer, the coated metal layer comprises a heat-conducting metal layer and an inert material coating on the heat-conducting metal layer, and the coated metal layer is sealably bonded to another layer of the substrate.    
     
     
         2 . The device of  claim 1 , further comprising detection reagents in the plurality of wells.  
     
     
         3 . The device of  claim 2 , wherein the detection reagents comprise reagents for polymerase chain reaction.  
     
     
         4 . The device of  claim 2 , wherein the detection reagents comprise probes and primers for polymerase chain reaction.  
     
     
         5 . The device of  claim 1 , wherein the inert material coating comprises a plastic polymer.  
     
     
         6 . The device of  claim 1 , wherein the heat-conducting metal layer comprises aluminum.  
     
     
         7 . The device of  claim 1 , wherein the heat-conducting metal layer comprises copper.