EPH-like receptor protein tyrosine kinases

Abstract

Four novel members of the EPH sub-family of receptor protein tyrosine kinases are disclosed. Nucleic acid sequences encoding receptor proteins, recombinant plasmids and host cells for expression, and methods of producing and using such receptors are also disclosed.

1 - 34 . (canceled)  
     
     
         35 . An isolated EPH-like receptor protein tyrosine kinase polypeptide or fragment thereof encoded by a nucleic acid selected from: 
 a) a nucleic acid comprising a nucleic acid selected from SEQ ID NO: 10 and SEQ ID NO: 12;    b) a nucleic acid comprising a portion of a nucleic acid selected from SEQ ID NO: 10 and SEQ ID NO: 12, wherein the portion encodes at least one of an extracellular domain and a cytoplasmic domain;    c) a nucleic acid hybridizing at 60° C. in 1M Na +  followed by washing at 60° C. in 0.2×SSC to the complement of the coding region of a nucleic acid selected from SEQ ID NO: 10 and SEQ ID NO: 12;    d) a nucleic acid comprising a portion of a nucleic acid hybridizing at 60° C. in 1M Na +  followed by washing at 60° C. in 0.2×SSC to the complement of the coding region of a nucleic acid selected from SEQ ID NO: 10 and SEQ ID NO: 12, wherein the portion encodes at least one of an extracellular domain and a cytoplasmic domain;    e) a nucleic acid that is 95% identical to a nucleic acid selected from SEQ ID NO: 10 and SEQ ID NO: 12;    f) a nucleic acid that is 95% identical to a portion of a nucleic acid selected from SEQ ID NO: 10 and SEQ ID NO: 12, wherein the portion encodes at least one of an extracellular domain and a cytoplasmic domain; and    g) a nucleic acid comprising a sequence which is degenerate to a nucleic acid of any one of (a)-(f).    
     
     
         36 . The isolated EPH-like receptor protein tyrosine kinase polypeptide or fragment thereof of  claim 35 , wherein the polypeptide or fragment thereof comprises an amino acid sequence selected from the amino acid sequence of SEQ ID NO: 11 and the amino acid sequence of SEQ ID NO: 13.  
     
     
         37 . The isolated EPH-like receptor protein tyrosine kinase polypeptide or fragment thereof of  claim 35 , wherein the polypeptide or fragment thereof comprises a portion of an amino acid sequence selected from the amino acid sequence of SEQ ID NO: 11 and the amino acid sequence of SEQ ID NO: 13, wherein the portion comprises at least one of an extracellular domain and a cytoplasmic domain.  
     
     
         38 . The isolated EPH-like receptor protein tyrosine kinase polypeptide or fragment thereof of  claim 37 , wherein the portion comprises an extracellular domain.  
     
     
         39 . The isolated EPH-like receptor protein tyrosine kinase polypeptide or fragment thereof of  claim 37 , wherein the polypeptide or fragment thereof comprises an amino acid sequence selected from amino acids 6 to 524 of SEQ ID NO: 11 and amino acids 1 to 547 of SEQ ID NO: 13.  
     
     
         40 . The isolated EPH-like receptor protein tyrosine kinase polypeptide or fragment thereof of  claim 38 , further comprising a heterologous receptor cytoplasmic domain.  
     
     
         41 . The isolated EPH-like receptor protein tyrosine kinase polypeptide or fragment thereof of  claim 39 , further comprising a heterologous receptor cytoplasmic domain.  
     
     
         42 . The polypeptide of  claim 35 , wherein the polypeptide is obtained by expression of a nucleic acid transformed or transfected into a procaryotic or eucaryotic host cell.