System, Method, and Computer Software Product for Gain Adjustment in Biological Microarray Scanner

Abstract

In accordance with some embodiments of the present invention, a method for gain adjustment is described that comprises (a) receiving a user selection of one or more parameters that includes a first resolution value; (b) obtaining a plurality of pixel intensity values using the user selected parameters and a gain value, wherein the obtained pixel intensity values are representative of a distribution of pixel intensity values; (c) generating a measure of comparison between the representative distribution and a desired distribution of pixel intensity values; (d) adjusting the gain value using the measure of comparison; and (e) obtaining a plurality of pixel intensity values using a second resolution value and the adjusted gain value.

1 . A method for gain adjustment, comprising: 
 (a) receiving a user selection of one or more parameters that includes a first resolution value;    (b) obtaining a plurality of pixel intensity values using the user selected parameters and a gain value, wherein the obtained pixel intensity values are representative of a distribution of pixel intensity values;    (c) generating a measure of comparison between the representative distribution and a desired distribution of pixel intensity values;    (d) adjusting the gain value using the measure of comparison; and    (e) obtaining a plurality of pixel intensity values using a second resolution value and the adjusted gain value.    
     
     
         2 . The method of  claim 1 , wherein: 
 the first resolution value includes a measure of resolution that is less than the second resolution value.    
     
     
         3 . The method of  claim 1 , wherein: 
 the first resolution value is indicative of an average value of two or more pixels.    
     
     
         4 . The method of  claim 1 , wherein: 
 the first resolution value is indicative of obtaining values from non-neighboring pixels, wherein one or more pixels are positioned between each pixel value obtained.    
     
     
         5 . The method of  claim 1 , wherein: 
 the first resolution value is indicative of obtaining lines of pixel values from non-neighboring lines of pixels, wherein one or more lines of pixels are positioned between each line of pixel values obtained.    
     
     
         6 . The method of  claim 1 , wherein: 
 the parameters include one or more values associated with a scan area.    
     
     
         7 . The method of  claim 6 , wherein: 
 the values associated with a scan area comprise a value associated with the X axis and a value associated with the Y axis.    
     
     
         8 . The method of  claim 7 , wherein: 
 the value associated with the X axis includes a measure of distance from a first point of reference, and the value associated with the Y axis includes a measure of distance from a first point of reference.    
     
     
         9 . The method of  claim 1 , further comprising: 
 repeating steps (b)-(d) using the adjusted gain value at each iteration until an acceptable gain value is determined; and    obtaining a plurality of pixel intensity values using a second resolution value and the acceptable gain value.    
     
     
         10 . A system for gain adjustment, comprising: 
 a gain data manager that receives a user selection of one or more parameters that includes a first resolution value;    an intensity manager that obtains a plurality of pixel intensity values using the user selected parameters and a gain value, wherein the obtained pixel intensity values are representative of a distribution of pixel intensity values;    a comparison manager that generates a measure of comparison between the representative distribution and a desired distribution of pixel intensity values;    a gain adjuster that adjusts the gain value using the measure of comparison; and    a controller that obtains a plurality of pixel intensity values using a second resolution value and the adjusted gain value.    
     
     
         11 . The system of  claim 10 , wherein: 
 the first resolution value includes a measure of resolution that is less than the second resolution value.    
     
     
         12 . The system of  claim 10 , wherein: 
 the first resolution value is indicative of an average value of two or more pixels.    
     
     
         13 . The system of  claim 10 , wherein: 
 the first resolution value is indicative of obtaining values from non-neighboring pixels, wherein one or more pixels are positioned between each pixel value obtained.    
     
     
         14 . The system of  claim 10 , wherein: 
 the first resolution value is indicative of obtaining lines of pixel values from non-neighboring lines of pixels, wherein one or more lines of pixels are positioned between each line of pixel values obtained.    
     
     
         15 . The method of  claim 10 , wherein: 
 the parameters include one or more values associated with a scan area.    
     
     
         16 . The method of  claim 15 , wherein: 
 the values associated with a scan area comprise a value associated with the X axis and a value associated with the Y axis.    
     
     
         17 . The method of  claim 16 , wherein: 
 the value associated with the X axis includes a measure of distance from a first point of reference, and the value associated with the Y axis includes a measure of distance from a first point of reference.