Mesoscale detection structures

Abstract

Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system further includes an analyte detection region in fluid communication with the flow channel comprised of a binding moiety for specifically binding the analyte. The detection region is constructed with a mesoscale dimension sufficiently small to enhance binding of the binding moiety and the analyte. The binding moiety may be immobilized in the detection region. The mesoscale detection systems of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, or for monitoring reactions or cell culture growth.

1 .- 42 . (canceled)  
     
     
         43 . A device comprising a first polymer substrate layer having a plurality of mesoscale channels fabricated thereon, which first polymer substrate layer is overlaid by a cover layer, which cover layer comprises a transparent portion, whereby the plurality of channels are sealed between the first polymer substrate layer and the cover layer.