Tumor specific monoclonal antibodies

Abstract

The invention provides tumor-specific human monoclonal antibodies and functional fragments. Also provided are nucleic acids encoding tumor-specific human monoclonal antibodies and functional fragments. A method for reducing neoplastic cell proliferation is also provided. The method consists of administering an effective amount of a tumor-specific human monoclonal antibody or functional fragment. Also provided is a method of detecting a neoplastic cell in a sample. The method consists of contacting a cell with a tumor-specific monoclonal antibody or functional fragment and detecting the specific binding of the human monoclonal antibody or functional fragment to the sample.

1 . An isolated human monoclonal antibody or functional fragment thereof comprising a light chain variable region and a heavy chain variable region with wherein the light chain variable region comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 28, 30, 32, 34, 36, 38, 40, 42 and 44, and wherein the heavy chain variable region comprises an amino acid sequence of SEQ ID NO: 2, and wherein said antibody or functional fragment thereof binds a neoplastic cell or antigen thereof.  
     
     
         2 . The isolated human monoclonal antibody of  claim 1 , wherein said functional fragment is selected from the group consisting of Fv, Fab, Fab′, and F(ab′) 2 .  
     
     
         3 . The isolated human monoclonal antibody or functional fragment of  claim 1 , further comprising a label.  
     
     
         4 . The isolated human monoclonal antibody or functional fragment of  claim 3 , wherein said label comprises a cytotoxic or cytostatic agent.  
     
     
         5 . A composition comprising an isolated human monoclonal antibody or functional fragment of  claim 1 , wherein the composition is physiologically acceptable and used for the treatment of cancer.