Method for vitrification of a biological specimen

Abstract

The present invention relates to a method of vitrification of a biological specimen. According to the method of the present invention, a biological specimen is directly exposed to a freezing material. Upon exposure to the freezing material, the biological specimen undergoes vitrification. The biological specimen which has undergone vitrification may be stored for a period of time, and then thawed at a later date. The thawed biological specimen remains viable. Preferred biological specimens according to the present invention are developmental cells.

1 . A system for the vitrification of a biological specimen, comprising: 
 (a) a loop;    (b) a base medium comprising a cryoprotectant held in the loop; and    (d) a thaw solution in contact with the loop.    
   
   
       2 . The system of  claim 1  wherein the loop holds a volume of 1-5 μl.  
   
   
       3 . The system of  claim 1  wherein the loop has a width of 20 μm.  
   
   
       4 . The system of  claim 1  wherein the loop has a diameter of 0.5 mm to 0.7 mm.