Cell separation method using alumina

Abstract

A method of separating cells or viruses using alumina is provided. The method includes: contacting a solution containing cells or viruses with alumina; and washing the alumina having cells or viruses bound thereto. According to the method, the separation efficiency of cells or viruses can be increased, the detection limit for cells or viruses can be improved, and a sample can be rapidly prepared.

1 . A method of separating cells or viruses using alumina, the method comprising: 
 contacting a solution containing cells or viruses with alumina; and    washing the alumina having cells or viruses bound thereto.    
     
     
         2 . A method of separating nucleic acids from cells or viruses using alumina, the method comprising: 
 contacting a solution containing cells or viruses with alumina;    washing the alumina having cells or viruses bound thereto; and    lysing the cells or viruses bound to the alumina.    
     
     
         3 . The method of  claim 1 , wherein the alumina is in the form of particle, membrane or substrate.  
     
     
         4 . The method of  claim 3 , wherein the alumina is prepared from aluminium ethoxide or aluminium chloride.  
     
     
         5 . The method of  claim 4 , wherein the alumina is prepared by sintering aluminium ethoxide or aluminium chloride at a temperature of 80-200° C.  
     
     
         6 . The method of  claim 1 , wherein the cells or viruses contact the alumina at pH 3-4.5.  
     
     
         7 . The method of  claim 2 , wherein the lysis of cells or viruses is carried out by electrolysis.  
     
     
         8 . The method of  claim 2 , further comprising directly performing a nucleic acid amplification using the separated nucleic acids.  
     
     
         9 . The method of  claim 8 , wherein the nucleic acid amplification is carried out by a PCR.  
     
     
         10 . The method of  claim 2 , wherein the alumina is in the form of particle, membrane or substrate.  
     
     
         11 . The method of  claim 10 , wherein the alumina is prepared from aluminium ethoxide or aluminium chloride.  
     
     
         12 . The method of  claim 11 , wherein the alumina is prepared by sintering aluminium ethoxide or aluminium chloride at a temperature of 80-200° C.  
     
     
         13 . The method of  claim 2 , wherein the cells or viruses contact the alumina at pH 3-4.5.