Polynucleotides encoding proteins involved in plant metabolism

Abstract

The invention provides isolated YABBY transcription factor nucleic acids and their encoded polypeptides. The present invention provides methods and compositions relating to altering YABBY transcription factor levels in plants. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.

1 . An isolated nucleic acid encoding a polypeptide selected from the group consisting of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, 308, 310, 312, 314, 316, 318, 320, 322, 324, 326, 328, 330, 332, 334, 336, 338, 340, 342, 344, 346, 348, 350, 352, 354, 356, 358, 360, and 362.  
     
     
         2 - 25 . (canceled)  
     
     
         26 . An isolated polynucleotide comprising: 
 (a) a nucleotide sequence encoding a YABBY transcription factor having an amino acid sequence of at least 90% sequence identity, based on the Clustal method of alignment with default pairwise alignment parameters of KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5, when compared to SEQ ID NO:130, or    (b) a complement of the nucleotide sequence of (a), wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.    
     
     
         27 . The polynucleotide of  claim 26 , wherein the amino acid sequence has at least 95% sequence identity, based on the Clustal method of alignment with the default pairwise alignment parameters, when compared to SEQ ID NO:130.  
     
     
         28 . The polynucleotide of  claim 26 , wherein the amino acid sequence comprises SEQ ID NO:130.  
     
     
         29 . The polynucleotide of  claim 26 , wherein the nucleotide sequence comprises SEQ ID NO:129.  
     
     
         30 . A vector comprising the polynucleotide of  claim 26 .  
     
     
         31 . A recombinant DNA construct comprising the polynucleotide of  claim 26  operably linked to at least one regulatory sequence.  
     
     
         32 . A method for transforming a cell, comprising transforming a cell with the polynucleotide of  claim 26 .  
     
     
         33 . A cell comprising the recombinant DNA construct of  claim 31 .  
     
     
         34 . A plant comprising the recombinant DNA construct of  claim 31 .  
     
     
         35 . A seed comprising the recombinant DNA construct of  claim 31.