Method for rapid amplification of DNA

Abstract

The present disclosure relates to methods of DNA amplification with a first primer that has a random sequence of nucleotides at its 3′ end and a generic sequence 5′ of the random nucleotides, as well as a second primer with the generic sequence of the first primer. The disclosure further relates to a method of precipitating DNA on a solid medium to improve DNA amplification. In a preferred embodiment, the presently disclosed methods are used for high-throughput genotyping of DNA samples, such as bloodstains or trace amounts of DNA.

1 - 70 . (canceled)  
     
     
         71 - 96 . (canceled)  
     
     
         97 . A method for preparing a DNA sample stored on a solid medium for amplification, comprising fixing said stored DNA sample to said solid medium with a solution comprising salt and alcohol, wherein said fixed DNA is suitable for amplification.  
     
     
         98 . A method for amplifying a DNA sample stored on a solid medium, comprising fixing said stored DNA sample to said solid medium with a solution comprising salt and alcohol, and amplifying said fixed DNA.  
     
     
         99 . The method of  claim 97  or  98 , wherein said solid medium is filter paper.  
     
     
         100 . The method of  claim 97  or  98 , wherein said filter paper is chemically treated.  
     
     
         101 . The method of  claim 97  or  98 , further comprising dehydrating said fixed DNA sample.  
     
     
         102 . The method of  claim 97  or  98 , wherein said DNA sample comprises tissue or blood.  
     
     
         103 . The method of  claim 97  or  98 , wherein said salt is an acetate salt or a chloride salt.  
     
     
         104 . The method of  claim 97  or  98 , wherein said alcohol is ethanol or isopropanol.