Enoyl-ACP reductases

Abstract

This invention relates to an isolated nucleic acid fragment encoding corn ( Zea maize ) and soybean ( Glycine max ) enoyl-ACP reductase. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the enoyl-ACP reductase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the enoyl-ACP reductase in a transformed host cell.

1 . An isolated polynucleotide comprising: 
 (a) a nucleotide sequence encoding a polypeptide having enoyl-ACP reductase activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 8 have at least 80% sequence identity, or    (b) the complement of the nucleotide sequence of (a).    
     
     
         2 . The polynucleotide of  claim 1 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 8 have at least 90% identity.  
     
     
         3 . The polynucleotide of  claim 1 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 8 have at least 95% identity.  
     
     
         4 . The polynucleotide of  claim 1 , wherein the amino acid sequence of the polypeptide comprises the amino acid sequence of SEQ ID NO: 8.  
     
     
         5 . The polynucleotide of  claim 1  wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO: 7.  
     
     
         6 . A vector comprising the polynucleotide of  claim 1 .  
     
     
         7 . A recombinant DNA construct comprising the polynucleotide of  claim 1  operably linked to at least one regulatory sequence.  
     
     
         8 . A method for transforming a cell, comprising transforming a cell with the polynucleotide of  claim 1 .  
     
     
         9 . A cell comprising the recombinant DNA construct of  claim 7 .  
     
     
         10 . A method for producing a plant comprising transforming a plant cell with the polynucleotide of  claim 1  and regenerating a plant from the transformed plant cell.  
     
     
         11 . A plant comprising the recombinant DNA construct of  claim 7 .  
     
     
         12 . A seed comprising the recombinant DNA construct of  claim 7 .  
     
     
         18 . The polynucleotide of  claim 1 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 8 have at least 85% identity.