Stably preserved microspheres

Abstract

In accordance with the present invention, active carboxylic acid ester groups are coupled on the surfaces of microspheres so as to reduce protocols for microsphere processing, control side reactions, and stably preserve beads containing active carboxylic acid ester groups. Further, microspheres labeled with at least one fluorescent dye cage in the microspheres, and the microspheres are preserved in lower alcohol.

1 . A microspheres, which is characterized in that microspheres labeled with at least one fluorescent dye caged in the microspheres, and the carrier microspheres is preserved in lower alcohol.  
   
   
       2 . The microspheres according to  claim 1 , which contains a functional group that activates a carboxyl group.  
   
   
       3 . The microspheres according to  claim 2 , in which the carboxyl group is activated to result in hydroxysuccinimide ester.  
   
   
       4 . The microspheres according to  claim 1 , in which the lower alcohol is isopropanol or  1 -butanol.  
   
   
       5 . The microspheres according to  claim 2 , in which the functional group that activates a carboxyl group is a succinimidyl ester group and/or a sulfo-succinimidyl ester group.  
   
   
       6 . The microspheres according to  claim 4 , in which the functional group that activates a carboxyl group is a succinimidyl ester group and/or a sulfo-succinimidyl ester group.  
   
   
       7 . The microspheres according to  claim 2 , in which the functional group that activates a carboxyl group is a 2,3,5,6-tetrafluorophenol ester group and/or a 4-sulfo-2,3,5,6-tetrafluorophenol ester group.  
   
   
       8 . The microspheres according to  claim 4 , in which the functional group that activates a carboxyl group is a 2,3,5,6-tetrafluorophenol ester group and/or a 4-sulfo-2,3,5,6-tetrafluorophenol ester group.