Methods and compositions for promoting wound healing

Abstract

The present invention provides methods and compositions that use the combination of Tris and EDTA to inhibit the growth of microorganisms at the site of a wound or burn, and/or to promote the healing of a wound or burn, and/or to reduce the sensation of pain at the site of a wound or burn. The amount of Tris and EDTA applied to a wound or burn can be selected to achieve one or more of the foregoing effects.

1 - 57 . (canceled)  
   
   
       58 . A liquid composition comprising Tris, EDTA, and a pharmaceutically acceptable carrier, wherein the Tris is present at a concentration in the range of from 10 mM to 250 mM, and the EDTA is present at a concentration in the range of from 1 mM to 250 mM.  
   
   
       59 . A composition of  claim 58  wherein the Tris is present at a concentration of from 10 mM to 80 mM, and the EDTA is present at a concentration in the range of from 1 mM to 50 mM.  
   
   
       60 . A composition of  claim 58  wherein the Tris is present at a concentration of from 10 mM to 50 mM, and the EDTA is present at a concentration in the range of from 1 mM to 20 mM.  
   
   
       61 . A composition of  claim 58  wherein the pharmaceutically acceptable carrier is selected from the group consisting of saline, physiological saline, ointment, cream, gel, and emulsion.  
   
   
       62 . A composition of  claim 58  wherein the Tris and EDTA are present in amounts that produce a pH of the composition of between 6.0 and 8.0 in aqueous solution.  
   
   
       63 . A composition of  claim 58  wherein the Tris and EDTA are present in amounts that produce a pH of the composition of between 6.5 and 7.5 in aqueous solution.  
   
   
       64 . A composition of  claim 58  further comprising a detergent.  
   
   
       65 . A composition of  claim 64  wherein the detergent is present in an amount of from 1% (v/v) to 30% (v/v).  
   
   
       66 . A composition of  claim 64  wherein the detergent is selected from the group consisting of cocamidopropyl betaine, cocamphodiacetate, sodium lauryl sulfate, sodium alkyl ether sulfate, lauramide DEA, and C 14 -C 16  olefin sulfonate.  
   
   
       67 . A composition of  claim 64  wherein the detergent consists essentially of cocamidopropyl betaine.  
   
   
       68 . A composition of  claim 58  further comprising an additional antimicrobial agent.  
   
   
       69 . A composition of  claim 58  wherein the additional antimicrobial agent is selected from the group consisting of an antibacterial agent and an antifungal agent.  
   
   
       70 - 80 . (canceled)