Application of lipid vehicles and use for drug delivery

Abstract

The present invention relates to compositions and methods for the administration of lipid-based vehicles to treat various disorders, including bladder inflammation, infection, dysfunction, and cancer. In various aspects, the compositions and methods of the invention are useful for prolonged delivery of drugs, e.g., antibiotics, pain treatments, and anticancer agents, to the bladder, genitourinary tract, gastrointestinal system, pulmonary system, and other organs or body systems. In particular, the present invention relates to liposome-based delivery of vanilloid compounds, such as resiniferatoxin, capsaicin, or tinyatoxin, and toxins, such as botulinum toxin, for the treatment of bladder conditions, including pain, inflammation, incontinence, and voiding dysfunction. Further related are methods of using these vehicles alone or in conjunction with antibodies, e.g., uroplakin antibodies, to improve duration of liposome attachment, and provide a long-term intravesical drug delivery platform. The present invention specifically relates to antibody-coated liposomes that are useful for targeting specific receptors for drug, peptide, polypeptide, or nucleic acid delivery. In one particular aspect, the present invention relates to liposomes coated with antibodies against nerve growth factor (NGF) receptor and containing NGF antisense nucleic acids, which are used as a treatment for neurogenic bladder dysfunction.

1 - 59 . (canceled)  
     
     
         60 . A method of treating bladder conditions comprising administering via intravesical instillation, to a mammalian subject in need thereof, an effective amount of a pharmaceutical composition, wherein the pharmaceutical composition comprises: 
 a) a non-ionic lipid vehicle, optionally containing an additional drug therein; and    b) a physiologically acceptable carrier, excipient, or diluent.    
     
     
         61 . The method of treating bladder conditions according to  claim 60 , wherein said non-ionic lipid vehicle contains an additional drug therein.  
     
     
         62 . The method of treating bladder conditions according to  claim 61 , wherein said drug comprises a botulinum toxin or a vanilloid.  
     
     
         63 . The method of treating bladder conditions according to  claim 62 , wherein said drug is selected from the group consisting of a botulinum toxin, resiniferatoxin, capsaicin and tinyatoxin.  
     
     
         64 . The method of treating bladder conditions according to  claim 63 , wherein said drug is administered in an amount effective to treat a condition selected from the group consisting of bladder pain, bladder inflammation, incontinence, voiding disfunction, urethral dyssynergia and bladder spasticity.