Koji mold-origin phospholipase a2

Abstract

It is intended to provide koji mold-origin phospholipase A 2 and a DNA encoding it. Namely, phospholipase A 2 comprising the following protein (a) or (b): (a) a protein having an amino acid sequence represented by SEQ ID NO: 1 or 2; and (b) a protein having an amino acid sequence derived from an amino acid sequence represented by SEQ ID NO: 1 or 2 by a partial modification and serving as phospholipase A 2 .

1 - 17 . (canceled)  
     
     
         18 . Phospholipase A 2  consisting of a protein selected from the group consisting of the following (a) to (d): 
 (a) a protein having an amino acid sequence set forth in SEQ ID NO: 1;    (b) a protein having an amino acid sequence obtained by modifying a part of the amino acid sequence set forth in SEQ ID NO: 1 and functioning as phospholipase A 2 ;    (c) a protein having an amino acid sequence set forth in SEQ ID NO: 2; and    (d) a protein having an amino acid sequence obtained by modifying a part of the amino acid sequence set forth in SEQ ID NO: 2, and functioning as phospholipase A 2 .    
     
     
         19 . A DNA selected from the group consisting of the following (A) and (B): 
 (A) a DNA encoding phospholipase A 2  described in claim  1 ; and    (B) a DNA which hybridizes under stringent conditions to the DNA described in (A) and encodes a protein functioning as phospholipase A 2 .    
     
     
         20 . A DNA according to claim  2 , having any one of the following sequences (i) to (vi): 
 (i) a base sequence of SEQ ID NO: 3;    (ii) a base sequence of SEQ ID NO: 4;    (iii) a base sequence of SEQ ID NO: 5;    (iv) a base sequence of SEQ ID NO: 6;    (v) a base sequence of SEQ ID NO: 7; and    (vi) a base sequence of SEQ ID NO: 8.    
     
     
         21 . A vector carrying the DNA according to claim  2 .  
     
     
         22 . A vector carrying the DNA according to claim  3 .  
     
     
         23 . Filamentous fungi into which the DNA according to claim  2  is exogenously introduced.  
     
     
         24 . Filamentous fungi into which the DNA according to claim  3  is exogenously introduced.  
     
     
         25 . A method for producing phospholipase A 2 , the method comprising the following steps (1) and (2): 
 (1) a step of culturing the filamentous fungi according to claim  6  under conditions where a protein encoded by the DNA can be produced; and    (2) a step of recovering the produced protein.    
     
     
         26 . A method for producing phospholipase A 2 , the method comprising the following steps (1) and (2): 
 (1) a step of culturing the filamentous fungi according to claim  7  under conditions where a protein encoded by the DNA can be produced; and    (2) a step of recovering the produced protein.