US2007020730A1PendingUtilityA1

Method for overexpression of zwitterionic polysaccharides

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Assignee: BRIGHAM & WOMENS HOSPITALPriority: Mar 13, 2002Filed: Sep 26, 2006Published: Jan 25, 2007
Est. expiryMar 13, 2022(expired)· nominal 20-yr term from priority
Inventors:Laurie ComstockMichael CoyneDennis L. KasperArthur O. TzianabosKatja Weinacht
A61P 37/02A61P 29/00A61P 1/00C07K 2319/00C12P 19/04C07K 14/195
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Claims

Abstract

The present invention is directed to methods for producing and selecting novel mutant strains of B. fragilis that constitutively express a particular capsular polysaccharide or only selected capsular polysaccharides; compositions directed to the novel mutant strains of B. fragilis that constitutively express a particular capsular polysaccharide or only selected capsular polysaccharides; improved methods for purification of individual capsular polysaccharides; and compositions directed to novel res02 and inv19 genes and their gene products. Significantly, the present invention provides methods and compositions for overexpressing and purifying immunomodulatory capsular polysaccharide A (PSA) in high yield.

Claims

exact text as granted — not AI-modified
1 - 28 . (canceled)  
     
     
         29 . A population of bacterial cells stably expressing a specific capsular polysaccharide selected from the group consisting of: PSA, PSB, PSD, PSE, PSF, PSG, and PSH.  
     
     
         30 . The population of bacterial cells according to  claim 29 , wherein the specific capsular polysaccharide is PSA.  
     
     
         31 . The population of bacterial cells according to  claim 29  or  claim 30 , wherein the bacterial cells are  B. fragilis.    
     
     
         32 . The population of bacterial cells according to  claim 29  or  claim 30 , wherein the bacterial cells are  B. fragilis  NCTC9343.  
     
     
         33 . The population of bacterial cells according to  claim 29  or  claim 30 , wherein the bacterial cells are  B. fragilis  9343res02mut44.  
     
     
         34 . The population of bacterial cells according to  claim 29  or  claim 30 , wherein the bacterial cells are  B. fragilis  9343res02mut2.  
     
     
         35 - 65 . (canceled)  
     
     
         66 . The population of bacterial cells according to  claim 30 , wherein the bacterial cells are  B. fragilis.    
     
     
         67 . The population of bacterial cells according to  claim 30 , wherein the bacterial cells are  B. fragilis  NCTC9343.  
     
     
         68 . The population of bacterial cells according to  claim 30 , wherein the bacterial cells are  B. fragilis  9343res02mut44.  
     
     
         69 . The population of bacterial cells according to  claim 30 , wherein the bacterial cells are  B. fragilis  9343res02mut2.

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