Method for breeding tomatoes having reduced water content and product of the method

Abstract

A method for breeding tomato plants that produce tomatoes with reduced fruit water content including the steps of crossing at least one Lycopersicon esculentum plant with a Lycopersicon spp. to produce hybrid seed, collecting the first generation of hybrid seeds, growing plants from the first generation of hybrid seeds, pollinating the plants of the most recent hybrid generation, collecting the seeds produced by the most recent hybrid generation, growing plants from the seeds of the most recent hybrid generation, allowing plants to remain on the vine past the point of normal ripening, and screening for reduced fruit water content as indicated by extended preservation of the ripe fruit and wrinkling of the fruit skin.

1 - 16 . (canceled)  
   
   
       17 . A method of producing tomato fruit capable of natural dehydration comprising: 
 (a) crossing at least one  Lycopersicon esculentum  plant with a  Lycopersicon  spp. to produce hybrid plants; and subsequently    (b) self-crossing and/or back-crossing said hybrid plants of step (a); and subsequently    (c) growing said hybrid plants of step (b) such that the fruit remains on the vine of said hybrid plants past normal red ripe harvest stage; and subsequently    (d) screening said hybrid plants of step (c) and isolating plants having fruit exhibiting a wrinkling phenotype, thereby producing tomato fruit capable of natural dehydration.    
   
   
       18 . The method according to  claim 17 , further comprising harvesting said tomato fruit following fruit wrinkling.  
   
   
       19 . A method of producing tomato fruit capable of natural dehydration comprising seeding tomato seeds, said tomato seeds directly or indirectly derived from said plants having said fruit exhibiting said wrinkling phenotype of  claim 17 , thereby producing tomato fruit capable of natural dehydration.  
   
   
       20 . A tomato plant obtained by the method of  claim 17 .  
   
   
       21 . An isolated tomato fruit obtained from the plant of  claim 20 .  
   
   
       22 . An isolated tomato seed obtained from the isolated tomato fruit of  claim 21 .  
   
   
       23 . A tomato plant, at least one ancestor thereof being the tomato plant of  claim 20 .  
   
   
       24 . An isolated tomato fruit obtained from the plant of  claim 23 .  
   
   
       25 . An isolated tomato seed obtained from the isolated tomato fruit of  claim 24 .  
   
   
       26 . An isolated whole tomato fruit comprising a genome of the  Lycopersicon esculentum  species, wherein said genome comprises an introgression from  Lycopersicon  spp., said introgression allowing natural fruit dehydration which results in skin wrinkling of the tomato fruit.  
   
   
       27 . An isolated whole tomato fruit comprising a genome of the  Lycopersicon esculentum  species, wherein said genome comprises an introgression from  Lycopersicon  spp., said introgression causing untreated skin wrinkling of the tomato fruit.