Support for analyte determination methods and method for producing the support

Abstract

A method for producing a support for determining analytes. The method comprises the steps of (a) providing a support comprising at least one channel, comprising a conduit having an intake and an outlet for passing fluid from the intake to the outlet, in the support body, (b) passing liquid with building blocks for synthesizing polymeric receptors through the channel or channels of the support body, (c) site- and/or time-specifically immobilizing the receptor building blocks in each case on predetermined positions in the channel or channels by illumination and (d) repeating steps (b) and (c) until the required receptors have been synthesized in each case on the predetermined positions.

1 . A method for producing a support for determining an analyte, comprising: 
 (a) providing an unlabelled support body    (b) inserting the support body into a synthesis unit,    (c) obtaining information from a database about sequences of receptors to be synthesized on the support,    (d) passing liquid containing receptor building blocks over the support body,    (e) site- or/and time-specific immobilising of the receptor building blocks at respective predetermined positions on the support body and    (f) repeating steps (d) to (e), until the desired receptors have been synthesized at the respective predetermined positions.    
     
     
         2 . The method of  claim 1  wherein the unlabelled support body of step (a) is provided by a manufacturer and is delivered to the owner of the synthesis unit of (b).  
     
     
         3 . The method of  claim 1  wherein in step (c) the sequences are taken from a freely accessible database.  
     
     
         4 . The method of  claim 1 , wherein in step (c) the sequences are taken from a database provided by the manufacturer of the unlabelled support body of (a).  
     
     
         5 . The method of  claim 1 , further comprising using the support for determining analytes in a sample.  
     
     
         6 . The method as claimed in  claim 5 , further comprising carrying out a plurality of synthesis/analyte determination cycles wherein the receptors for a subsequent cycle are synthesized on the basis of the information from a preceding cycle.  
     
     
         7 . The method of  claim 5 , wherein the sample is derived from a patient.  
     
     
         8 . The method of  claim 7 , further comprising adjusting the results of the determination to the individual differences of the patient and from the results, forming a second support on which a determination adapted to the individual patient is carried out.  
     
     
         9 . The method of  claim 5 , further comprising inserting the support body into an integrated synthesis and analysis unit, contacting the carrier, after the synthesis of the receptors, with a sample containing analytes and determining the analytes by their binding to the receptors immobilized on the support.  
     
     
         10 . The method of  claim 1 , wherein the support body comprises at least one channel.  
     
     
         11 . The method of  claim 1 , wherein the support body comprises a plurality of channels.  
     
     
         12 . The method of  claim 11 , wherein the channels are assembled parallel to one another.  
     
     
         13 . The method of  claim 1 , wherein the receptors are selected from nucleic acids and nucleic acid analogues.  
     
     
         14 . The method of  claim 13 , wherein the receptor building blocks are selected from nucleosides, oligonucleotides, nucleotide analogues and oligonucleotide analogues.  
     
     
         15 . The method of  claim 1 , wherein the receptors are selected from polypeptides.  
     
     
         16 . The method of  claim 15 , wherein the receptor building blocks are selected from amino acids and peptides.  
     
     
         17 . The method of  claim 1 , further comprising providing a reagent comprising a support and building blocks for the synthesis of polymeric receptors on the support.  
     
     
         18 . The method of  claim 1 , further comprising inserting the support body into a synthesis unit comprising a programmable light source matrix, a detector matrix, a means for supplying fluids to the support, and for discharging fluids from the support, and an electronic control and regulation means, wherein the support body is accommodated between the programmable light source matrix and the detector matrix.  
     
     
         19 . The method of  claim 1 , wherein the synthesis unit further comprises a means for deprotecting reaction components on the support.  
     
     
         20 . The method of  claim 1  further comprising sequencing nucleic acids.  
     
     
         21 . The method of  claim 20  wherein said sequencing is new sequencing.  
     
     
         22 . The method of  claim 21 , wherein the genetic material is selected from the group consisting of individual genomes and synthetic nucleic acids.  
     
     
         23 . The method of  claim 1  further comprising obtaining diagnostic information.  
     
     
         24 . The method of  claim 24 , wherein the diagnostic information is the individual effect of pharmaceuticals.  
     
     
         25 . The method of  claim 24  further comprising managing individual patients.  
     
     
         26 . The method of  claim 1  further comprising analyzing the effect of pharmacological substances.  
     
     
         27 . The method of  claim 1  further comprising manufacturing and analyzing substance libraries.  
     
     
         28 . The method of  claim 1  further comprising comparing individuals in a population.  
     
     
         29 . A system for integrated synthesis of receptors on a support, comprising: 
 (a) a support body,    (b) a synthesis unit,    (c) a means for the synthesis of receptors by step-by-step assembly of receptor building blocks at respective predetermined positions on the support and    (d) a database for obtaining information about sequences of receptors to be synthesized on the support.    
     
     
         30 . The system of  claim 30 , wherein the support body comprises at least one channel.  
     
     
         31 . The system of  claim 30 , wherein the synthesis unit comprises a programmable light source matrix, a detector matrix, a means for supplying fluids to the support, and for discharging fluids from the support, and an electronic control and regulation means.  
     
     
         32 . The system according to  claim 31 , wherein the support body is arranged between the programmable light source matrix and the detector matrix.  
     
     
         33 . The method of  claim 20  wherein said sequencing is resequencing complex genetic material.