Method of weak partitioning chromatography

Abstract

This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.

1 . A method of recovering a purified product from a load fluid, comprising the steps of: 
 passing the load fluid through a medium at operating conditions which cause the medium to bind at least 1 mg of product per 1 mL of medium; and    recovering the purified product from column effluent.    
   
   
       2 . A method of recovering a purified product from a load fluid, comprising the steps of: 
 passing the load fluid through a medium at operating conditions defined by a partition coefficient of at least 0.1; and    recovering the purified product from column effluent.    
   
   
       3 . The method of  claim 1 , wherein the operating conditions cause the medium to bind at least 10 mg of product per mL of medium.  
   
   
       4 . The method of  claim 2 , wherein the value of the partition coefficient is in the range of about 0.2 to about 10.0.  
   
   
       5 . The method of  claim 1 , further comprising the step of recovering the purified product from the medium using an essentially isocratic wash.  
   
   
       6 . The method of  claim 2 , further comprising the step of recovering the purified product from the medium using an essentially isocratic wash.  
   
   
       7 . The method of  claim 1 , wherein the purified product is a protein selected from the group consisting of fusion proteins, Fc-containing proteins, immunoconjugates, cytokines, interleukins, hormones, and therapeutic enzymes.  
   
   
       8 . The method of  claim 2 , wherein the purified product is a protein selected from the group consisting of fusion proteins, Fc-containing proteins, immunoconjugates, cytokines, interleukins, hormones, and therapeutic enzymes.  
   
   
       9 . The method of  claim 1 , wherein the operating conditions comprise a pH of 7.5-8.2 and a chloride concentration of 12 mM-55 mM.  
   
   
       10 . The method of  claim 2 , wherein the operating conditions comprise a pH of 7.5-8.2 and a chloride concentration of 12 mM-55 mM.