Methods for selective targeting

Abstract

A selective targeting method is disclosed which comprises contacting a peptide library with an anti-target to allow the peptides to bind; separating non-binding peptides from the anti-target bound peptides, contacting the non-binding anti-target peptides with a target allowing the unbound peptides to bind with the target to form a target-bound peptide complex; separating the target-bound peptide complex from peptides which do not bind to the target, and identifying the target-bound peptides. In one embodiment the target is skin or hair. In another embodiment the anti-target is hair when the target is skin, and the anti-target is skin when the target is hair.

1 . A method for screening a peptide library comprising the steps of, 
 (a) contacting the peptide library with an anti-target to allow the peptides to bind with said anti-target;    (b) separating unbound peptides;    (c) contacting the unbound peptides with a selected target to allow said unbound peptides to bind with the target to form a target-bound peptide complex;    (d) separating said target-bound peptide complex from peptides which do not bind to said target; and    (e) identifying the target-bound peptides on the target-bound peptide complex, wherein said anti-target is skin or hair and said target is skin when the anti-target is hair or said target is hair when the anti-target is skin.    
     
     
         2 . The method according to  claim 1 , wherein step (a), (b), (c) or (d) is repeated between 2 to 10 times.  
     
     
         3 . (canceled)  
     
     
         4 . The method according to  claim 1 , wherein the identifying step comprises amplifying a nucleic acid coding for the target-bound peptide in a polymerase chain reaction.  
     
     
         5 . The method according to  claim 1 , wherein the target-bound peptide is not released from the target during the identifying step.  
     
     
         6 . The method according to  claim 1 , wherein the peptides are fused to a phage coat protein.  
     
     
         7 . The method according to  claim 1 , wherein separating said target-bound peptide further includes an acid elution step.  
     
     
         8 - 12 . (canceled)