Novel peptide, novel adsorbent, adsorption unit and adsorption method

Abstract

The present invention provides an adsorbent, adsorption unit, and an adsorption method which make it possible to selectively adsorb an immunoglobulin and/or an immune complex present in a body fluid (for example, blood, plasma, etc.) efficiently without pretreating the body fluid. By immobilizing a compound having binding activity for an immunoglobulin and/or an immune complex on a water-insoluble carrier, an adsorbent having remarkably excellent adsorption capacity may be obtained.

1 . A peptide having affinities for an immunoglobulin and/or an immune complex which comprises the following amino acid sequence shown under the SEQ ID NO: 1 of the sequence listing.  
       
         
           
                 
                 
               
                     
                 
                   Thr Thr Tyr Lys Leu Val Ile Asn Gly Lys Thr Leu 
                     
                 
                     
                 
                   Lys Gly Glu Thr Thr Thr Lys Ala Val Asp Ala Glu 
                 
                     
                 
                   Thr Ala Glu Arg Ala Phe Arg X01 Tyr Ala X02 Asp 
                 
                     
                 
                   Asn Gly Val X03 Gly X04 Trp Thr Tyr Asp Pro Ala 
                 
                     
                 
                   Thr Lys Thr Phe Thr Val Thr Glu Cys 
                 
                     
                 
                     wherein,    
                 
                     X01: Asn, Lys, or Gln    
                 
                     X02: Thr or Asn    
                 
                     X03: Glu or Asp    
                 
                     X04: Leu, Val, Ile, or Met.    
                 
                     
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         2 . A nucleotide sequence which codes for at least one species of the amino acid sequence shown under the SEQ ID NO: 1 of the sequence listing.  
     
     
         3 . The nucleotide sequence according to  claim 2  which comprises at least one species of the base sequence shown under the SEQ ID NO:2 of the sequence listing.  
     
     
         4 . A recombinant DNA which has one or more nucleotide sequences coding for the amino acid sequence shown under the SEQ ID NO: 1 of the sequence listing.  
     
     
         5 . The recombinant DNA according to  claim 4 , wherein the nucleotide sequence comprises at least one species of the base sequence shown under the SEQ ID NO:2 of the sequence listing.  
     
     
         6 . The recombinant DNA according to  claim 4 , which is a plasmid or a phage.  
     
     
         7 . A microorganism which comprises at least one recombinant DNA having one or more nucleotide sequences coding for the amino acid sequence shown under the SEQ ID NO: 1 of the sequence listing.  
     
     
         8 . The microorganism according to  claim 7 , wherein the nucleotide sequence comprises at lease one species of the base sequence shown under the SEQ ID NO:2 of the sequence listing.  
     
     
         9 . The recombinant DNA according to  claim 5 , which is a plasmid or a phage.