US2007065838A1PendingUtilityA1

Oligonucleotide mediated nucleic acid recombination

Assignee: MAXYGEN INCPriority: Jan 19, 1999Filed: Mar 23, 2006Published: Mar 22, 2007

Est. expiryJan 19, 2019(expired)· nominal 20-yr term from priority

Inventors:Andreas Crameri Willem P. C. Stemmer Jeremy Minshull Steven H. Bass Mark Welch John E. Ness Claes Gustafsson Phillip A. Patten

C12P 21/06

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References

Claims

Abstract

Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.

Claims

exact text as granted — not AI-modified

1 . A method of recombining homologous nucleic acids, the method comprising: 
 (i) hybridizing a set of family gene shuffling oligonucleotides; and,    (ii) elongating the set of family gene shuffling oligonucleotides, thereby providing a population of recombined nucleic acids.    
     
     
         2 - 104 . (canceled)

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