Sugar and lipid metabolism regulators in plants III

Abstract

Isolated nucleic acids and proteins associated with lipid and sugar metabolism regulation are provided. In particular, lipid metabolism proteins (LMP) and encoding nucleic acids originating from Arabidopsis thaliana are provided. The nucleic acids and proteins are used in methods of producing transgenic plants and modulating levels of seed storage compounds. Preferably, the seed storage compounds are lipids, fatty acids, starches or seed storage proteins.

1 - 42 . (canceled)  
     
     
         43 . A method of producing a transgenic plant having seed that is true breeding for an increased level of total fatty acids as compared to seed of a wild type variety of the plant, the method comprising the steps of: 
 a) transforming a plant cell with an expression vector comprising an isolated nucleic acid encoding a full-length cytidilate kinase polypeptide, said nucleic acid comprising a polynucleotide having a sequence selected from the group consisting of: 
 i) a polynucleotide having the sequence as set forth in SEQ ID NO:9;  
 ii) a polynucleotide encoding a polypeptide having the sequence as set forth in SEQ ID NO:10;  
 iii) a polynucleotide that hybridizes under stringent conditions to the polynucleotide having the sequence as set forth in SEQ ID NO:9;  
 iv) a polynucleotide that hybridizes under stringent conditions to the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10;  
 v) a polynucleotide having at least 95% sequence identity with the polynucleotide having the sequence as set forth in SEQ ID NO:9;  
 vi) a polynucleotide having at least 95% sequence identity with the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10; and  
 vii) a polynucleotide complementary to the polynucleotide of any of i) through vi) above; and  
   b) generating from the transformed plant cell a transgenic plant that expresses the nucleic acid;    wherein the stringent conditions comprise the steps of hybridization in a 6× sodium chloride/sodium citrate (SSC) solution at about 45° C., followed by at least one wash in a solution of 0.2×SSC and 0.1% sodium dodecyl sulfate at 65° C.    
     
     
         44 . The method of  claim 43 , wherein the nucleic acid comprises the polynucleotide having the sequence as set forth in SEQ ID NO:9.  
     
     
         45 . The method of  claim 43 , wherein the nucleic acid comprises the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10.  
     
     
         46 . The method of  claim 43 , wherein the nucleic acid comprises the polynucleotide that hybridizes under stringent conditions to the polynucleotide having the sequence as set forth in SEQ ID NO:9.  
     
     
         47 . The method of  claim 43 , wherein the nucleic acid comprises the polynucleotide that hybridizes under stringent conditions to the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10.  
     
     
         48 . The method of  claim 43 , wherein the nucleic acid comprises the polynucleotide having at least 95% sequence identity with the polynucleotide having the sequence as set forth in SEQ ID NO:9.  
     
     
         49 . The method of  claim 43 , wherein the nucleic acid comprises the polynucleotide having at least 95% sequence identity with the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10.  
     
     
         50 . The method of  claim 43 , wherein the plant is a monocot.  
     
     
         51 . The method of  claim 43 , wherein the plant is a dicot.  
     
     
         52 . The method of  claim 43 , wherein the plant is selected from the group consisting of rapeseed, canola, linseed, soybean, sunflower, maize, oat, rye, barley, wheat, sugarbeet, tagetes, cotton, oil palm, coconut palm, flax, castor and peanut.  
     
     
         53 . The method of  claim 52 , wherein the plant is rapeseed or canola.  
     
     
         54 . The method of  claim 52 , wherein the plant is soybean.  
     
     
         55 . The method of  claim 52 , wherein the plant is corn.  
     
     
         56 . A transgenic plant having seed that is true breeding for an increased level of total fatty acids as compared to seed of a wild type variety of the plant, the transgenic plant produced by a method comprising the steps of: 
 a) transforming a plant cell with an expression vector comprising an isolated nucleic acid encoding a full-length cytidilate kinase polypeptide, said nucleic acid comprising a polynucleotide having a sequence selected from the group consisting of: 
 i) a polynucleotide having the sequence as set forth in SEQ ID NO:9;  
 ii) a polynucleotide encoding a polypeptide having the sequence as set forth in SEQ ID NO:10;  
 iii) a polynucleotide that hybridizes under stringent conditions to the polynucleotide having the sequence as set forth in SEQ ID NO:9;  
 iv) a polynucleotide that hybridizes under stringent conditions to the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10;  
 v) a polynucleotide having at least 95% sequence identity with the polynucleotide having the sequence as set forth in SEQ ID NO:9;  
 vi) a polynucleotide having at least 95% sequence identity with the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10; and  
 vii) a polynucleotide complementary to the polynucleotide of any of i) through vi) above; and  
   b) generating from the transformed plant cell a transgenic plant that expresses the nucleic acid;    wherein the stringent conditions comprise the steps of hybridization in a 6× sodium chloride/sodium citrate (SSC) solution at about 45° C., followed by at least one wash in a solution of 0.2×SSC and 0.1% sodium dodecyl sulfate at 65° C.    
     
     
         57 . The transgenic plant of  claim 56 , wherein the nucleic acid comprises the polynucleotide having the sequence as set forth in SEQ ID NO:9.  
     
     
         58 . The transgenic plant of  claim 56 , wherein the nucleic acid comprises the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10.  
     
     
         59 . The transgenic plant of  claim 56 , wherein the nucleic acid comprises the polynucleotide that hybridizes under stringent conditions to the polynucleotide having the sequence as set forth in SEQ ID NO:9.  
     
     
         60 . The transgenic plant of  claim 56 , wherein the nucleic acid comprises the polynucleotide that hybridizes under stringent conditions to the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10.  
     
     
         61 . The transgenic plant of  claim 56 , wherein the nucleic acid comprises the polynucleotide having at least 95% sequence identity with the polynucleotide having the sequence as set forth in SEQ ID NO:9.  
     
     
         62 . The transgenic plant of  claim 56 , wherein the nucleic acid comprises the polynucleotide having at least 95% sequence identity with the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10.  
     
     
         63 . The transgenic plant of  claim 56 , wherein the plant is a monocot.  
     
     
         64 . The transgenic plant of  claim 56 , wherein the plant is a dicot.  
     
     
         65 . The transgenic plant of  claim 56 , wherein the plant is selected from the group consisting of rapeseed, canola, linseed, soybean, sunflower, maize, oat, rye, barley, wheat, sugarbeet, tagetes, cotton, oil palm, coconut palm, flax, castor and peanut.  
     
     
         66 . The transgenic plant of  claim 56 , wherein the plant is rapeseed or canola.  
     
     
         67 . The transgenic plant of  claim 56 , wherein the plant is soybean.  
     
     
         68 . The transgenic plant of  claim 56 , wherein the plant is corn.  
     
     
         69 . A transgenic plant seed that is true breeding for an increased level of total fatty acids as compared to seed of a wild type variety of the plant, said transgenic plant seed being produced by a method comprising the steps of: 
 a) transforming a plant cell with an expression vector comprising an isolated nucleic acid encoding a full-length cytidilate kinase polypeptide, said nucleic acid comprising a polynucleotide having a sequence selected from the group consisting of: 
 i) a polynucleotide having the sequence as set forth in SEQ ID NO:9;  
 ii) a polynucleotide encoding a polypeptide having the sequence as set forth in SEQ ID NO:10;  
 iii) a polynucleotide that hybridizes under stringent conditions to the polynucleotide having the sequence as set forth in SEQ ID NO:9;  
 iv) a polynucleotide that hybridizes under stringent conditions to the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10;  
 v) a polynucleotide having at least 95% sequence identity with the polynucleotide having the sequence as set forth in SEQ ID NO:9;  
 vi) a polynucleotide having at least 95% sequence identity with the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10; and  
 vii) a polynucleotide complementary to the polynucleotide of any of i) through vi) above;  
   b) generating from the transformed plant cell a transgenic plant that expresses the nucleic acid; and    c) harvesting transgenic seed from the transformed plant;    wherein the stringent conditions comprise the steps of hybridization in a 6× sodium chloride/sodium citrate (SSC) solution at about 45° C., followed by at least one wash in a solution of 0.2×SSC and 0.1% sodium dodecyl sulfate at 65° C.    
     
     
         70 . The transgenic plant seed of  claim 69 , wherein the nucleic acid comprises the polynucleotide having the sequence as set forth in SEQ ID NO:9.  
     
     
         71 . The transgenic plant seed of  claim 69 , wherein the nucleic acid comprises the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10.  
     
     
         72 . The transgenic plant seed of  claim 69 , wherein the nucleic acid comprises the polynucleotide that hybridizes under stringent conditions to the polynucleotide having the sequence as set forth in SEQ ID NO:9.  
     
     
         73 . The transgenic plant seed of  claim 69 , wherein the nucleic acid comprises the polynucleotide that hybridizes under stringent conditions to the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10.  
     
     
         74 . The transgenic plant seed of  claim 69 , wherein the nucleic acid comprises the polynucleotide having at least 95% sequence identity with the polynucleotide having the sequence as set forth in SEQ ID NO:9.  
     
     
         75 . The transgenic plant seed of  claim 69 , wherein the nucleic acid comprises the polynucleotide having at least 95% sequence identity with the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10.  
     
     
         76 . The transgenic plant seed of  claim 69 , wherein the plant is a monocot.  
     
     
         77 . The transgenic plant seed of  claim 69 , wherein the plant is a dicot.  
     
     
         78 . The transgenic plant seed of  claim 69 , wherein the plant is selected from the group consisting of rapeseed, canola, linseed, soybean, sunflower, maize, oat, rye, barley, wheat, sugarbeet, tagetes, cotton, oil palm, coconut palm, flax, castor and peanut.  
     
     
         79 . The transgenic plant seed of  claim 78 , wherein the plant is rapeseed or canola.  
     
     
         80 . The transgenic plant seed of  claim 78 , wherein the plant is soybean.  
     
     
         81 . The transgenic plant seed of  claim 78 , wherein the plant is corn.  
     
     
         82 . A method of increasing fatty acid content of a plant seed, the method comprising the steps of: 
 a) transforming a plant cell with an expression vector comprising an isolated nucleic acid encoding a full-length cytidilate kinase polypeptide, said nucleic acid comprising a polynucleotide having a sequence selected from the group consisting of: 
 i) a polynucleotide having the sequence as set forth in SEQ ID NO:9;  
 ii) a polynucleotide encoding a polypeptide having the sequence as set forth in SEQ ID NO:10;  
 iii) a polynucleotide that hybridizes under stringent conditions to the polynucleotide having the sequence as set forth in SEQ ID NO:9;  
 iv) a polynucleotide that hybridizes under stringent conditions to the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10;  
 v) a polynucleotide having at least 95% sequence identity with the polynucleotide having the sequence as set forth in SEQ ID NO:9;  
 vi) a polynucleotide having at least 95% sequence identity with the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10; and  
 vii) a polynucleotide complementary to the polynucleotide of any of i) through vi) above;  
   b) generating from the transformed plant cell a transgenic plant that expresses the nucleic acid; and    c) harvesting transgenic seed that has increased fatty acid content from the transgenic plant;    wherein the stringent conditions comprise the steps of hybridization in a 6× sodium chloride/sodium citrate (SSC) solution at about 45° C., followed by at least one wash in a solution of 0.2×SSC and 0.1% sodium dodecyl sulfate at 65° C.    
     
     
         83 . The method of  claim 82 , wherein the nucleic acid comprises the polynucleotide having the sequence as set forth in SEQ ID NO:9.  
     
     
         84 . The method of  claim 82 , wherein the nucleic acid comprises the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10.  
     
     
         85 . The method of  claim 82 , wherein the nucleic acid comprises the polynucleotide that hybridizes under stringent conditions to the polynucleotide having the sequence as set forth in SEQ ID NO:9.  
     
     
         86 . The method of  claim 82 , wherein the nucleic acid comprises the polynucleotide that hybridizes under stringent conditions to the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10.  
     
     
         87 . The method of  claim 82 , wherein the nucleic acid comprises the polynucleotide having at least 95% sequence identity with the polynucleotide having the sequence as set forth in SEQ ID NO:9.  
     
     
         88 . The method of  claim 82 , wherein the nucleic acid comprises the polynucleotide having at least 95% sequence identity with the polynucleotide encoding the polypeptide having the sequence as set forth in SEQ ID NO:10.  
     
     
         89 . The method of  claim 82 , wherein the plant is a monocot.  
     
     
         90 . The method of  claim 82 , wherein the plant is a dicot.  
     
     
         91 . The method of  claim 82 , wherein the plant is selected from the group consisting of rapeseed, canola, linseed, soybean, sunflower, maize, oat, rye, barley, wheat, sugarbeet, tagetes, cotton, oil palm, coconut palm, flax, castor and peanut.  
     
     
         92 . The method of  claim 91 , wherein the plant is rapeseed or canola.  
     
     
         93 . The method of  claim 91 , wherein the plant is soybean.  
     
     
         94 . The method of  claim 92 , wherein the plant is corn.