Oncoprotein protein kinase

Abstract

An isolated polypeptide (JNK) characterized by having a molecular weight of 46 kD as determined by reducing SDS-PAGE, having serine and Threonine kinase activity, phosphorylating the c-Jun N-terminal activation domain and polynucleotide sequences and method of deterction of JNK are provided herein. JNK phosphorylates c-Jun N-terminal activation domain which affects gene expression from AP-1 sites.

1 . A kit for use in detecting c-Jun N-terminal kinase comprising: 
 at least one nucleic acid probe that hybridizes to a polynucleotide encoding the kinase under stringent conditions; and    at least one a reporter-means for detecting the polynucleotide.    
     
     
         2 . The kit of  claim 1 , wherein the probe hybridizes to SEQ ID NO: 13 under stringent conditions.  
     
     
         3 . The kit of  claim 1 , wherein the probe hybridizes to SEQ ID NO: 14 under stringent conditions.  
     
     
         4 . The kit of  claim 1 , wherein the kinase is encoded by a nucleic acid which hybridizes to the complement of SEQ ID NO: 11 under stringent conditions.  
     
     
         5 . The kit of  claim 1 , wherein the kinase is encoded by a nucleic acid which hybridizes to the complement of SEQ ID NO: 17 under stringent conditions.  
     
     
         6 . The kit of  claim 1 , wherein the reporter-means is a biotin-binding protein.  
     
     
         7 . The kit of  claim 6 , wherein the biotin-binding protein is avidin or streptavidin.  
     
     
         8 . The kit of  claim 1 , further comprising at least one buffer.