Polypeptides of Alicyclobacillus sp.

Abstract

The present invention relates to Isolated mature functional polypeptide which is at least 90% identical to and exhibits the same function of a corresponding secreted polypeptide obtainable from the bacterium Alicyclobacillus sp. deposited under accession number DSM 15716 are disclosed.

1 . An isolated mature functional polypeptide which is at least 90% identical to and exhibits the same function of a corresponding secreted polypeptide obtainable from the bacterium  Alicyclobacillus  sp. deposited under accession number DSM 15716.  
     
     
         2 . (canceled)  
     
     
         3 . The polypeptide of  claim 1  selected from the group consisting of: 
 (a) a polypeptide comprising an amino acid sequence which has at least 90% identity with a sequence of a mature polypeptide comprised in the group of SEQ ID NO: 26 to SEQ ID NO: 50;    (b) a polypeptide which is encoded a nucleotide sequence which hybridize under high stringency conditions with a polynucleotide probe selected from the group consisting of 
 (i) the complementary strand to a nucleotide sequence selected from the group of regions of SEQ ID NO: 1 to SEQ ID NO: 25 encoding a mature polypeptide.  
 (ii) the complementary strand to the cDNA sequence contained in a nucleotide sequences selected from the group of regions of SEQ ID NO: 1 to SEQ ID NO: 25 encoding a mature polypeptide;  
   (c) a fragment of a mature polypeptide comprised in SEQ ID NO: 26 to SEQ ID NO: 50 and    wherein the polypeptide has a function of the corresponding mature polypeptides comprised in SEQ ID NO: 26 to SEQ ID NO: 50.    
     
     
         4 - 62 . (canceled)  
     
     
         63 . An isolated bacterial glutamic peptidase (EC 3.4.23.19).  
     
     
         64 . The glutamic peptidase of  claim 63  selected from the group consisting of: 
 (a) a polypeptide comprising an amino acid sequence which has at least 90% identity with a sequence of a mature polypeptide secreted from the strain of  Alicyclobacillus  sp. deposited under DSM accession No. 15716 or comprised in SEQ ID NO: 27;    (b) a polypeptide which is encoded a nucleotide sequence which hybridize under high stringency conditions with a polynucleotide probe selected from the group consisting of 
 (i) the complementary strand to the region of SEQ ID NO: 2 encoding a mature polypeptide or the complementary strand to a nucleotide sequence comprised in the strain of  Alicyclobacillus  sp. deposited under DSM accession No. 15716 encoding a mature glutamic peptidase secreted from that strain;  
 (ii) the complementary strand to the cDNA sequence contained in the region of SEQ ID NO: 2 encoding a mature polypeptide or the complementary strand to a cDNA sequence comprised in the strain of  Alicyclobacillus  sp. deposited under DSM accession No. 15716 encoding a mature glutamic peptidase secreted from that strain; and  
   (c) a fragment of a mature glutamic peptidase comprised in SEQ ID NO: 27;    wherein the glutamic peptidase has a function of the corresponding mature glutamic peptidase comprised in SEQ ID NO: 27.    
     
     
         65 . The glutamic peptidase of  claim 64 , wherein the stringency conditions are very high.  
     
     
         66 . The glutamic peptidase of  claim 63 , wherein the polynucleotide encoding the glutamic peptidase consists of the region of SEQ ID NO: 2 encoding a mature glutamic peptidase or a sequence differing there from by virtue of the degeneracy of the genetic code.  
     
     
         67 . The glutamic peptidase of  claim 63 , which is free of disulphide bridges in the peptidase structure.  
     
     
         68 . The glutamic peptidase of  claim 63 , which is obtained from the strain of  Alicyclobacillus  sp. deposited under DSM accession No. 15716.  
     
     
         69 . The glutamic peptidase of  claim 63 , comprising or consisting of the mature glutamic peptidase comprised in SEQ ID NO: 27.  
     
     
         70 . The glutamic peptidase enzyme of  claim 69 , comprising or consisting of the sequences from positions 1 to 240 of SEQ ID NO: 27.  
     
     
         71 . A composition comprising the glutamic peptidase of  claim 63 .  
     
     
         72 . The composition of  claim 71 , further comprising at least two different polypeptides, preferably at least 3, more preferable at least 5, more preferable at least 10, more preferable at least 15, more preferable at least 20 different polypeptides, preferably all polypeptides secreted when fermenting a sample of  Alicyclobacillus  sp. DSM 15716 or a mutant thereof wherein one or more genes has been deleted or added.  
     
     
         73 . The composition of  claim 71 , further comprising one or more additional enzymes.  
     
     
         74 . The composition of  claim 71 , which is a detergent composition which further comprises a surfactant.  
     
     
         75 . The composition of  claim 75 , which is a feed composition which further comprises a cereal or grain product.  
     
     
         76 . The composition of  claim 71 , which is a food composition.  
     
     
         77 . The composition of  claim 71 , further comprising a polysaccharide or a mixture of polysaccharides.  
     
     
         78 . A method for preparing a composition of  claim 71 , comprising admixing the glutamic peptidase of  claim 63  with an excipient.  
     
     
         79 . A process comprising employing a glutamic peptidase of  claim 63  in an industrial or household technical process.  
     
     
         80 . A polynucleotide having a nucleotide sequence which encodes for the glutamic peptidase of  claim 63 .  
     
     
         81 . A nucleic acid construct comprising the nucleotide sequence of  claim 80  operably linked to one or more control sequences that direct the production of the glutamic peptidase in a host cell.  
     
     
         82 . A recombinant expression vector comprising the nucleic acid construct of  claim 81 .  
     
     
         83 . A recombinant host cell comprising the nucleic acid construct of  claim 81 .  
     
     
         84 . A method for producing a glutamic peptidase of  claim 1 , comprising: 
 (a) cultivating a strain, which in its wild-type form is capable of producing the glutamic peptidase, to produce the glutamic peptidase; and    (b) recovering the glutamic peptidase.    
     
     
         85 . A method for producing a glutamic peptidase, comprising: 
 (a) cultivating a recombinant host cell of  claim 83  under conditions conducive for production of the glutamic peptidase; and    (b) recovering the glutamic peptidase.    
     
     
         86 . An isolated bacterial strain deposited under accession number DSM 15716.