Matrices for drug delivery and methods for making and using the same

Abstract

In one aspect, biocompatible matrices such as sol-gels encapsulating a reaction center may be administered to a subject for conversion of prodrugs into biologically active agents. In certain embodiments, the biocompatible matrices of the present invention are sol-gels. In one embodiment, the enzyme L -amino acid decarboxylase is encapsulated and implanted in the brain to convert L -dopa to dopamine for treatment of Parkinson's disease.

1 - 138 . (canceled)  
     
     
         139 . A method of toxicology testing, comprising: 
 a. encapsulating at least one reaction center in a silica-based sol-gel matrix;    b. interacting a compound with said matrix; and,    c. evaluating for any products of said compound resulting from conversion of said compound by said reaction center,    wherein production of any cytotoxic or mutagenic products indicates that said compound may be toxic to a subject upon administration.    
     
     
         140 . The method of  claim 139 , wherein said reaction center comprises an enzyme that is located in the liver of a mammal.  
     
     
         141 . The method of  claim 139 , wherein said reaction center is an enzyme prepared by recombinant methods.  
     
     
         142 . The method of  claim 139 , wherein said reaction center is cell-free.  
     
     
         143 . The method of  claim 140 , wherein said mammal is one of the following: a pig or a human.