Human Protease Molecules

Abstract

The invention provides human protease molecules (HUPM) and polynucleotides which identify and encode HUPM. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for treating or preventing disorders associated with expression of HUPM.

1 . (canceled)  
     
     
         2 . An isolated antibody which specifically binds to a polypeptide selected from the group consisting of: 
 a) a polypeptide comprising an amino acid sequence of SEQ ID NO:6,    b) a polypeptide comprising a naturally occurring amino acid sequence at least 90% identical to an amino acid sequence of SEQ ID NO:6,    c) a biologically active fragment of a polypeptide having an amino acid sequence of SEQ ID NO:6, and    d) an immunogenic fragment of a polypeptide having an amino acid sequence of SEQ ID NO:6.    
     
     
         3 . A diagnostic test for a condition or disease associated with the expression of HUPM in a biological sample, the method comprising: 
 a) combining the biological sample with an antibody of  claim 2 , under conditions suitable for the antibody to bind the polypeptide and form an antibody polypeptide complex, and    b) detecting the complex, wherein the presence of the complex correlates with the presence of the polypeptide in the biological sample.    
     
     
         4 . The antibody of  claim 2 , wherein the antibody is: 
 a) a chimeric antibody,    b) a single chain antibody,    c) a Fab fragment,    d) a F(ab′) 2  fragment, or    e) a humanized antibody.    
     
     
         5 . A composition comprising an antibody of  claim 2  and an acceptable excipient.  
     
     
         6 . A method of diagnosing a condition or disease associated with the expression of HUPM in a subject, comprising administering to said subject an effective amount of the composition of  claim 5 .  
     
     
         7 . A composition of  claim 5 , wherein the antibody is labeled.  
     
     
         8 . A method of diagnosing a condition or disease associated with the expression of HUPM in a subject, comprising administering to said subject an effective amount of the composition of  claim 7 .  
     
     
         9 . A method of preparing a polyclonal antibody with the specificity of the antibody of  claim 2 , the method comprising: 
 a) immunizing an animal with a polypeptide having an amino acid sequence of SEQ ID NO:6, or an immunogenic fragment thereof, under conditions to elicit an antibody response,    b) isolating antibodies from said animal, and    c) screening the isolated antibodies with the polypeptide, thereby identifying a polyclonal antibody which binds specifically to a polypeptide having an amino acid sequence of SEQ ID NO:6.    
     
     
         10 . An antibody produced by a method of  claim 9 .  
     
     
         11 . A composition comprising the antibody of  claim 10  and a suitable carrier.  
     
     
         12 . A method of making a monoclonal antibody with the specificity of the antibody of  claim 2 , the method comprising: 
 a) immunizing an animal with a polypeptide having an amino acid sequence of SEQ ID NO:6, or an immunogenic fragment thereof, under conditions to elicit an antibody response,    b) isolating antibody producing cells from the animal,    c) fusing the antibody producing cells with immortalized cells to form monoclonal antibody-producing hybridoma cells,    d) culturing the hybridoma cells, and    e) isolating from the culture monoclonal antibody which binds specifically to a polypeptide having an amino acid sequence of SEQ ID NO:6.    
     
     
         13 . A monoclonal antibody produced by a method of  claim 12 .  
     
     
         14 . A composition comprising the antibody of  claim 13  and a suitable carrier.  
     
     
         15 . The antibody of  claim 2 , wherein the antibody is produced by screening a Fab expression library.  
     
     
         16 . The antibody of  claim 2 , wherein the antibody is produced by screening a recombinant immunoglobulin library.  
     
     
         17 . A method of detecting a polypeptide having an amino acid sequence of SEQ ID NO:6 in a sample, the method comprising: 
 a) incubating the antibody of  claim 2  with a sample under conditions to allow specific binding of the antibody and the polypeptide, and    b) detecting specific binding, wherein specific binding indicates the presence of a polypeptide having an amino acid sequence of SEQ ID NO:6 in the sample.    
     
     
         18 . A method of purifying a polypeptide having an amino acid sequence of SEQ ID NO:6 from a sample, the method comprising: 
 a) incubating the antibody of  claim 2  with a sample under conditions to allow specific binding of the antibody and the polypeptide, and    b) separating the antibody from the sample and obtaining the purified polypeptide having an amino acid sequence selected of SEQ ID NO:6.