Image based correction for unwanted light signals in a specific region of interest

Abstract

A method for correcting the signal in an image having a plurality of regions of interest, the method comprising the steps of: (a) providing an image having a plurality of regions of interest, these regions of interest having areas between them; (b) determining a region of correction between at least two regions of interest; (c) calculating a correction signal from the region of correction; and (d) using the correction signal to correct a measured signal from one or more regions of interest. This invention also provides a method for defining a region of correction for use in a method for correcting the signal in an image having a plurality of regions of interest, the defining method comprising the steps of: (a) providing an image having a plurality of regions of interest; (b) extracting geometric information for a plurality of regions of interest; (c) selecting a location between at least two regions of interest; (d) selecting at least one parameter to describe regions of correction; and (e) constructing regions of correction between the at least two regions of interest.

1 . A method for correcting the signal in an image having a plurality of regions of interest, the method comprising the steps of: 
 (a) providing an image having a plurality of regions of interest, these regions of interest having areas between them;    (b) determining a region of correction between at least two regions of interest;    (c) calculating a correction signal from the region of correction; and    (d) using the correction signal to correct a signal measurement from one or more regions of interest.    
   
   
       2 . The method of  claim 1 , further including the step of determining a background signal and adjusting the correction signal of a run by subtracting the background signal from the correction signal.  
   
   
       3 . The method of  claim 2 , wherein the background signal is a background signal stored prior to commencing the run.  
   
   
       4 . The method of  claim 2 , wherein the background signal is a background signal determined during the run.  
   
   
       5 . The method of  claim 1 , wherein the correction signal is scaled.  
   
   
       6 . The method of  claim 1 , wherein the regions of correction have a plurality of sides.  
   
   
       7 . The method of  claim 1 , wherein the regions of correction have four sides.  
   
   
       8 . The method of  claim 1 , wherein the regions of correction are closed polygons.  
   
   
       9 . The method of  claim 1 , wherein the regions of correction are circular.  
   
   
       10 . The method of  claim 1 , wherein the regions of correction are annular.  
   
   
       11 . The method of  claim 1 , wherein the regions of correction are defined by a bitmap.  
   
   
       12 . The method of  claim 1 , wherein said plurality of regions of interest are from a multi-well plate.  
   
   
       13 . The method of  claim 1 , wherein a thermocycler reader is employed.  
   
   
       14 . The method of  claim 1 , further including the step of storing the regions of correction defined in step (c).  
   
   
       15 . A method for defining a region of correction for use in a method for correcting the signal in an image having a plurality of regions of interest, the defining method comprising the steps of: 
 (a) providing an image having a plurality of regions of interest;    (b) extracting geometric information for the plurality of regions of interest;    (c) selecting a location between at least two regions of interest;    (d) selecting at least one parameter to describe regions of correction; and    (e) constructing regions of correction between the at least two regions of interest.    
   
   
       16 . The method of  claim 15 , further including the step of constructing additional regions of correction that are not between the at least two regions of interest.  
   
   
       17 . The method of  claim 16 , wherein a sufficient number of additional regions of correction are constructed so that each region of interest has the same number of regions of correction as does any other region of interest.  
   
   
       18 . The method of  claim 15 , wherein the geometric information for the regions of interest is a centroid.  
   
   
       19 . The method of  claim 18 , wherein the location between at least two regions of interest is the center point between the centroids of at least two regions of interest.  
   
   
       20 . The method of  claim 15 , wherein the regions of correction are selected from the group consisting of polygons, circles, annuli, and bitmaps.  
   
   
       21 . The method of  claim 15 , further including the step of storing the regions of correction associated with the at least two regions of interest.