Antibody and antibody fragments for inhibiting the growth of tumors

Abstract

Chimerized and humanized versions of anti EGF receptor antibody 225 and fragments thereof for treatment of tumors.

1 . A polypeptide lacking the constant region and the variable light chain of an antibody, the polypeptide comprising the amino acid sequence N Y G V H, G VI W S G G N T D Y N T P F T S R, OR V I W S G G N T D Y N T P F T S.  
     
     
         2 . A polypeptide according to  claim 1 , comprising amino acid sequences N Y G V H and G V I W S G N N T D Y N T P F T S R or V I W S G G N T D Y N T P F T S.  
     
     
         3 . A polypeptide consisting of the amino acid sequence N Y G V H or G V I W S G G N T D Y N T P F T S R.  
     
     
         4 . A polypeptide consisting of the amino acid sequence N Y G V H or V I W S G G N T D Y N T P F T S.  
     
     
         5 . A polypeptide according to  claim 1  conjugated to an effector molecule.  
     
     
         6 - 13 . (canceled)  
     
     
         14 . DNA encoding a polypeptide lacking the constant region and the variable light chain of an antibody, the polypeptide comprising the amino acid sequence N Y G V H, G V I W S G G N T D Y N T P F T S R or V I W S G G N T D Y N T P F T S.  
     
     
         15 - 17 . (canceled)  
     
     
         18 . A molecule having the constant region of a human antibody and the hyper variable region of monoclonal antibody 225 conjugated to an effector molecule.  
     
     
         19 - 22 . (canceled)  
     
     
         23 . A molecule comprising: a constant region of a human antibody; a variable region other than the CDRs of a human antibody, the variable region comprising a kappa light chain and a heavy chain, and the CDRs of monoclonal antibody 225.  
     
     
         24 - 45 . (canceled)  
     
     
         46 . A method for significantly inhibiting the growth of tumor cells in a human comprising administering to the human an effective amount of the polypeptide according to  claim 1 .  
     
     
         47 - 83 . (canceled)  
     
     
         84 . A nucleic acid molecule that encodes a molecule comprising: a constant region of a human antibody: a variable region other than the CDRs of a humen antibody, the variable region comprising a kappa light chain and a heavy chain, and the cDRs of monoclonal antibody 225.  
     
     
         85 . A vector comprising the nucleic acid molecule of  claim 84 .  
     
     
         86 - 88 . (canceled)  
     
     
         89 . A prokaryotic cell comprising the vector of  claim 85 .  
     
     
         90 . A eukaryotic cell comprising the vector of claim  88 .  
     
     
         91 . A pharmaceutical composition, comprising the molecule of  claim 23  and a pharmaceutically acceptable carrier.