US2007122388A1PendingUtilityA1

Tissue graft compositions and methods for producing same

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Assignee: KROPP BRADLEY PPriority: Dec 8, 2000Filed: Dec 19, 2006Published: May 31, 2007
Est. expiryDec 8, 2020(expired)· nominal 20-yr term from priority
Inventors:Bradley KroppHsueh-Kung LinFadee Mondelek
A61L 27/3679A61L 27/3813A61L 27/383A61L 2400/12A61F 2/02C12N 5/0685A61L 27/3886A61L 27/3882A61K 35/12C12N 2502/1347C12N 2531/00A61L 27/3826C12N 2533/92C12N 2533/40A61F 2/04A61K 35/38A61L 2430/22A61L 27/3629
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Claims

Abstract

A tissue graft composition is described herein that includes a segment of small intestinal submucosa having at least one nanoparticle incorporated therein such that the permeability of the segment of small intestinal submucosa is altered, thereby providing the segment of small intestinal submucosa with a more substantially uniform structure for cell migration and proliferation. The tissue graft composition may further comprise at least one macromolecule incorporated into the nanoparticle. The tissue graft composition may be utilized in seeded or unseeded methods of tissue repair.

Claims

exact text as granted — not AI-modified
1 . A tissue graft composition, comprising: 
 a segment of small intestinal submucosa; and    at least one nanoparticle incorporated into the segment of small intestinal submucosa.    
     
     
         2 . The tissue graft composition of  claim 1 , wherein the segment of small intestinal submucosa consists essentially of a distal ileal segment of small intestinal submucosa isolated from a mature adult pig.  
     
     
         3 . The tissue graft composition of  claim 1 , wherein the at least one nanoparticle has a size from about 200 to about 500 nanometers.  
     
     
         4 . The tissue graft composition of  claim 1 , wherein the tissue graft composition has decreased permeability when compared to the segment of small intestinal submucosa alone.  
     
     
         5 . The tissue graft composition of  claim 1 , wherein the at least one nanoparticle has at least one macromolecule incorporated therein, wherein the at least one macromolecule is selected from the group consisting of growth factors, hormones, nucleic acids, polysaccharides, drugs, and combinations thereof.  
     
     
         6 . The tissue graft composition of  claim 1 , wherein the at least one nanoparticle is selected from the group consisting of poly(lactic-co-glycolic) acid (PLGA) nanoparticles, poly lactic acid (PLA) nanoparticles, chitosen nanoparticles, liposomes, and combinations thereof.  
     
     
         7 . The tissue graft composition of  claim 1 , further defined as a urinary tract tissue graft composition.  
     
     
         8 . The tissue graft composition of  claim 7 , further comprising at least one cell type seeded on a surface of the segment of small intestinal submucosa, wherein the at least one cell type is selected from the group consisting of smooth muscle cells, urothelial cells and stem cells.  
     
     
         9 . The tissue graft composition of  claim 8 , wherein smooth muscle cells are seeded on a mucosal surface of the segment of small intestinal submucosa, and urothelial cells are seeded on a serosal surface of the segment of small intestinal submucosa.  
     
     
         10 . The tissue graft composition of  claim 8 , wherein smooth muscle cells are seeded on a mucosal surface of the segment of small intestinal submucosa, and wherein urothelial cells are seeded upon the smooth muscle cells on the mucosal surface of the segment of small intestinal submucosa.  
     
     
         11 . A kit, comprising: 
 a tissue graft composition, comprising a segment of small intestinal submucosa and at least one nanoparticle incorporated into the segment of small intestinal submucosa; and    means for suspending the tissue graft composition and holding the tissue graft composition in a taut position such that cells may be seeded thereon.    
     
     
         12 . The kit of  claim 11 , wherein the means is a tissue culture frame.  
     
     
         13 . The kit of  claim 11 , wherein the segment of small intestinal submucosa consists essentially of a distal ileal segment of small intestinal submucosa isolated from a mature adult pig.  
     
     
         14 . The kit of  claim 11 , wherein the at least one nanoparticle has a size from about 200 to about 500 nanometers.  
     
     
         15 . The kit of  claim 11 , wherein the tissue graft composition has decreased permeability when compared to the segment of small intestinal submucosa alone.  
     
     
         16 . The kit of  claim 11 , wherein the at least one nanoparticle has at least one macromolecule incorporated therein, wherein the at least one macromolecule is selected from the group consisting of growth factors, hormones, nucleic acids, polysaccharides, drugs, and combinations thereof.  
     
     
         17 . The kit of  claim 11 , wherein the at least one nanoparticle is selected from the group consisting of poly(lactic-co-glycolic) acid (PLGA) nanoparticles, poly lactic acid (PLA) nanoparticles, chitosen nanoparticles, liposomes, and combinations thereof.  
     
     
         18 . A method for repairing a damaged tissue of a subject, comprising the steps of: 
 providing a tissue graft composition comprising a segment of small intestinal submucosa having a mucosal surface and a serosal surface and at least one nanoparticle incorporated into the segment of small intestinal submucosa;    contacting the damaged tissue with the tissue graft composition under conditions such that growth of the tissue occurs and the damaged tissue is repaired, thereby restoring function to the tissue.    
     
     
         19 . The method of  claim 18  wherein, in the step of providing a tissue graft composition, the segment of small intestinal submucosa consists essentially of a distal ileal segment of small intestinal submucosa isolated from a mature adult pig.  
     
     
         20 . The method of  claim 18  wherein, in the step of providing a tissue graft composition, the least one nanoparticle has a size from about 200 to about 500 nanometers.  
     
     
         21 . The method of  claim 18  wherein, in the step of providing a tissue graft composition, the tissue graft composition has decreased permeability when compared to the segment of small intestinal submucosa alone.  
     
     
         22 . The method of  claim 18  wherein, in the step of providing a tissue graft composition, the at least one nanoparticle has at least one macromolecule incorporated therein, wherein the at least one macromolecule is selected from the group consisting of growth factors, hormones, nucleic acids, polysaccharides, drugs, and combinations thereof.  
     
     
         23 . The method of  claim 18  wherein, in the step of providing a tissue graft composition, the at least one nanoparticle is selected from the group consisting of poly(lactic-co-glycolic) acid (PLGA) nanoparticles, poly lactic acid (PLA) nanoparticles, chitosen nanoparticles, liposomes, and combinations thereof.  
     
     
         24 . The method of  claim 18  further comprising the steps of: 
 isolating and culturing at least one cell type from a tissue specimen of a subject;    seeding the at least one cell type on the segment of small intestinal submucosa; and    allowing the segment of small intestinal submucosa having the at least one cell type seeded thereon to mature in culture such that the at least one cell type exhibits three dimensional growth and matrix penetrance, prior to contacting the damaged tissue with the segment of small intestinal submucosa.    
     
     
         25 . The method of  claim 24 , wherein the at least one cell type is selected from the group consisting of smooth muscle cells and urothelial cells.  
     
     
         26 . The method of  claim 24 , wherein the damaged tissue is a damaged urinary tract tissue.

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