Gankyrin

Abstract

Gankyrin having the amino acid sequence as set forth in SEQ ID NO: 2, or modified gankyrin comprising an amino acid sequence modified by the deletion and/or addition of one or a plurality of amino acids and/or the substitution with other amino acids in the amino acid sequence of SEQ ID NO: 2 and retaining the biological activity of gankyrin, a gene encoding it, and a method of preparing said protein and uses thereof.

1 . An agent for treating cancer comprising an inhibitor to a polypeptide having a biological activity of gankyrin (gankyrin inhibitor).  
     
     
         2 . An agent for treating hepatoma comprising an inhibitor to a polypeptide having a biological activity of gankyrin.  
     
     
         3 . An agent for treating cancer comprising a gankyrin inhibitor which inhibits a function of a polypeptide having a biological activity of gankyrin.  
     
     
         4 . An agent for treating hepatoma comprising an gankyrin inhibitor which inhibits a function of a polypeptide having a biological activity of gankyrin.  
     
     
         5 . An agent for treating cancer comprising a gankyrin inhibitor which inhibits an expression of a polypeptide having a biological activity of gankyrin.  
     
     
         6 . An agent for treating hepatoma comprising an gankyrin inhibitor which inhibits an expression of a polypeptide having a biological activity of gankyrin.  
     
     
         7 . An agent for treating cancer according to  claim 3 , wherein the gankyrin inhibitor which inhibits a function of a polypeptide having a biological activity of gankyrin is an antibody to a polypeptide having a biological activity of gankyrin.  
     
     
         8 . An agent for treating hepatoma according to  claim 4 , wherein the gankyrin inhibitor which inhibits a function of a polypeptide having a biological activity of gankyrin is an antibody to a polypeptide having a biological activity of gankyrin.  
     
     
         9 . An agent for treating cancer according to  claim 5 , wherein the gankyrin inhibitor which inhibits an function of a polypeptide having a biological activity of gankyrin is an antisense oligonucleotide to a gene encoding a polypeptide having a biological activity of gankyrin.  
     
     
         10 . An agent for treating hepatoma according to  claim 6 , wherein the gankyrin inhibitor which inhibits an function of a polypeptide having a biological activity of gankyrin is an antisense oligonucleotide to a gene encoding a polypeptide having a biological activity of gankyrin.  
     
     
         11 . An agent for treating cancer according to  claim 7 , wherein the antibody is a monoclonal antibody.  
     
     
         12 . An agent for treating hepatoma according to  claim 8 , wherein the antibody is a monoclonal antibody.  
     
     
         13 . An agent for treating cancer according to  claim 7 , wherein the antibody is a humanized antibody.  
     
     
         14 . An agent for treating hepatoma according to  claim 8 , wherein the antibody is a humanized antibody.  
     
     
         15 . An agent for treating cancer according to  claim 9 , wherein the antisense oligonucleotide is an oligoncleotide hybridizing to a part of the nucleotide sequence shown in SEQ ID NO: 1.  
     
     
         16 . An agent for treating cancer according to  claim 9 , wherein the antisense oligonucleotide is an oligoncleotide hybridizing to a continuous at least 20 nucleotides in the nucleotide sequence shown in SEQ ID NO: 1.  
     
     
         17 . An agent for treating cancer according to  claim 16 , wherein the continuous at least 20 nucleotides contain a translation start codon.  
     
     
         18 . An agent for treating cancer according to  claim 1 , wherein the polypeptide having a biological activity of gankyrin is selected from the group consisting of: 
 (a) A polypeptide comprising an amino acid sequence from Ala at position 14 to Gly at position 226 of SEQ ID NO: 2 and having the biological activity of gankyrin;    (b) A polypeptide comprising an amino acid sequence modified by the deletion and/or addition of one or a plurality of amino acids and/or the substitution with other amino acids in the amino acid sequence from Ala at position 14 to Gly at position 226 of SEQ ID NO: 2 and retaining the biological activity of gankyrin;    (c) A polypeptide comprising an amino acid sequence from Met at position 1 to Gly at position 226 of SEQ ID NO: 2 and having the biological activity of gankyrin;    (d) A polypeptide comprising an amino acid sequence modified by the deletion and/or addition of one or a plurality of amino acids and/or the substitution with other amino acids in the amino acid sequence from Met at position 1 to Gly at position 226 of SEQ ID NO: 2 and retaining the biological activity of gankyrin;    (e) A polypeptide that is encoded by a DNA capable of hybridizing under a stringent condition to a DNA having the nucleotide sequence as set forth in SEQ ID NO: 1 and that has the biological properties of gankyrin;    (f) A polypeptide comprising an amino acid sequence from Ala at position 14 to Met at position 231 of SEQ ID NO: 4 and having the biological activity of gankyrin;    (g) A polypeptide comprising an amino acid sequence modified by the deletion and/or addition of one or a plurality of amino acids and/or the substitution with other amino acids in the amino acid sequence from Ala at position 14 to Met at position 231 of SEQ ID NO: 4 and retaining the biological activity of gankyrin;    (h) A polypeptide comprising an amino acid sequence from Met at position 1 to Met at position 231 of SEQ ID NO: 4 and having the biological activity of gankyrin;    (i) A polypeptide comprising an amino acid sequence modified by the deletion and/or addition of one or a plurality of amino acids and/or the substitution with other amino acids in the amino acid sequence from Met at position 1 to Met at position 231 of SEQ ID NO: 4 and retaining the biological activity of gankyrin;    (j) A polypeptide that is encoded by a DNA capable of hybridizing under a stringent condition to a DNA having the nucleotide sequence as set forth in SEQ ID NO: 3 and that has the biological properties of gankyrin;    (k) A polypeptide comprising an amino acid sequence from Ala at position 14 to Met at position 231 of SEQ ID NO: 6 and having the biological activity of gankyrin;    (l) A polypeptide comprising an amino acid sequence modified by the deletion and/or addition of one or a plurality of amino acids and/or the substitution with other amino acids in the amino acid sequence from Ala at position 14 to Met at position 231 of SEQ ID NO: 6 and retaining the biological activity of gankyrin;    (m) A polypeptide comprising an amino acid sequence from Met at position 1 to Met at position 231 of SEQ ID NO: 6 and having the biological activity of gankyrin;    (n) A polypeptide comprising an amino acid sequence modified by the deletion and/or addition of one or a plurality of amino acids and/or the substitution with other amino acids in the amino acid sequence from Met at position 1 to Met at position 231 of SEQ ID NO: 6 and retaining the biological activity of gankyrin; and    (o) A polypeptide that is encoded by a DNA capable of hybridizing under a stringent condition to a DNA having the nucleotide sequence as set forth in SEQ ID NO: 5 and that has the biological properties of gankyrin.