Plant Myb Transcription Factor Homologs

Abstract

This invention relates to an isolated nucleic acid fragment encoding a Myb-related transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the Myb-related transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the Myb-related transcription factor in a transformed host cell.

1 . An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: 
 (a) a first nucleotide sequence encoding a polypeptide of at least 50 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 12, and SEQ ID NO:56;    (b) a second nucleotide sequence encoding a polypeptide of at least 50 amino acids that has at least 85% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NO: 8 and SEQ ID NO:28;    (c) a third nucleotide sequence encoding a polypeptide of at least 50 amino acids that has at least 90% identity based on the Clustal method of alignment when compared to a polypeptide of SEQ ID NO: 16;    (d) a fourth nucleotide sequence encoding a polypeptide of at least 50 amino acids that has at least 95% identity based on the Clustal method of alignment when compared to polypeptide of SEQ ID NO: 52;    (e) a fifth nucleotide sequence encoding a polypeptide of at least 100 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NO: 6, SEQ ID NO: 14, SEQ ID NO:50, and SEQ ID NO:58;    (f) a sixth nucleotide sequence encoding a polypeptide of at least 100 amino acids that has at least 85% identity based on the Clustal method of alignment when compared to SEQ ID NO: 60;    (g) a seventh nucleotide sequence encoding a polypeptide of at least 100 amino acids that has at least 90% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NO: 4, SEQ ID NO:10, SEQ ID NO:22, SEQ ID NO:24, and SEQ ID NO:62;    (h) a eighth nucleotide sequence encoding a polypeptide of at least 100 amino acids that has at least 95% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NO: 18 and SEQ ID NO:20;    (i) a ninth nucleotide sequence encoding a polypeptide of at least 150 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NO: 26, SEQ ID NO: 34, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:42, SEQ ID NO:48, and SEQ ID NO:54;    (j) a tenth nucleotide sequence encoding a polypeptide of at least 150 amino acids that has at least 85% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NO: 32, SEQ ID NO:44, and SEQ ID NO:46;    (k) an eleventh nucleotide sequence encoding a polypeptide of at least 200 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to SEQ ID NO: 36;    (l) a twelfth nucleotide sequence encoding a polypeptide of at least 200 amino acids that has at least 85% identity based on the Clustal method of alignment when compared to SEQ ID NO: 30; and    (m) a thirteenth nucleotide sequence comprising the complement of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k), or (l).    
     
     
         2 - 16 . (canceled)  
     
     
         17 . An isolated polynucleotide comprising: 
 (a) a nucleotide sequence encoding a polypeptide having Myb-related transcription factor activity, wherein the polypeptide has an amino acid sequence of at least 90% sequence identity when compared to SEQ ID NO:10, based on the Clustal V method of alignment with pairwise alignment default parameters of KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5, or    (b) a full-length complement of the nucleotide sequence of (a).    
     
     
         18 . The polynucleotide of  claim 17 , wherein the amino acid sequence of the polypeptide has at least 95% sequence identity when compared to SEQ ID NO:10, based on the Clustal V method of alignment with the pairwise alignment default parameters.  
     
     
         19 . The polynucleotide of  claim 17 , wherein the amino acid sequence of the polypeptide comprises SEQ ID NO:10.  
     
     
         20 . The polynucleotide of  claim 17  wherein the nucleotide sequence comprises SEQ ID NO:9.  
     
     
         21 . A vector comprising the polynucleotide of  claim 17 .  
     
     
         22 . A recombinant DNA construct comprising the polynucleotide of  claim 17  operably linked to at least one regulatory sequence.  
     
     
         23 . A method for transforming a cell, comprising transforming a cell with the polynucleotide of  claim 17 .  
     
     
         24 . A cell comprising the recombinant DNA construct of  claim 22 .  
     
     
         25 . A method for producing a transgenic plant comprising transforming a plant cell with the polynucleotide of  claim 17  and regenerating a transgenic plant from the transformed plant cell.  
     
     
         26 . A plant comprising the recombinant DNA construct of  claim 22 .  
     
     
         27 . A seed comprising the recombinant DNA construct of  claim 22.