Method for increasing an abiotic-resistance in monocot plant

Abstract

The present invention relates to a method for increasing resistance of monocot plants against abiotic stress which comprises a step of transforming monocot plants with a recombinant plasmid containing a fused gene (TPSP) of trehalose-6-phosphate synthetase (TPS) gene and trehalose-6-phosphate phosphatase (TPP) gene to express the TPSP gene while maintaining normal growth and development characteristics. The present invention can increase the resistance of monocot plants against various stresses so that it can greatly contribute to the improvement of production and quality of valuable agricultural crops.

1 . A method for increasing resistance of monocot plants against abiotic stress, which comprises a step of transforming a monocot plant with a recombinant plasmid containing a fused bifunctional fusion enzyme gene (TPSP) of trehalose-6-phosphate synthetase (TPS) gene and trehalose-6-phosphate phosphatase (TPP) gene to express the TPSP gene, thereby limiting trehalose-6-phosphate accumulation and enhancing accumulation of trehalose in the transformed monocot plants while maintaining normal plant growth and development characteristics.  
     
     
         2 . The method for increasing resistance of monocot plants against abiotic stresses according to  claim 1 , wherein TPS gene and TPP gene are derived from  E. coli  or yeast.  
     
     
         3 . The method for increasing resistance of monocot plants against abiotic stresses according to  claim 1 , wherein the monocot plant is rice, wheat, barley, wheat or maize.  
     
     
         4 . The method for increasing resistance of monocot plants against abiotic stresses according to  claim 1 , wherein the transformation is carried out according to  Agrobacterium -mediated method.  
     
     
         5 . The method for increasing resistance of monocot plants against abiotic stresses according to  claim 1 , wherein the abiotic stress is dehydration-stress, salt-stress or cold-stress.  
     
     
         6 . A method for producing monocot plants having increased resistance against abiotic stresses, which comprises a step of transforming monocot plants or their ancestors with a recombinant plasmid containing a fused bifunctional fusion enzyme gene (TPSP) of trehalose-6-phosphate synthetase (TPS) gene and trehalose-6-phosphate phosphatase (TPP) gene to express the TPSP gene, thereby limiting trehalose-6-phosphate accumulation and enhancing accumulation of trehalose in the transformed monocot plants to enable growth without phenotypic growth alteration.  
     
     
         7 . A method for generating a transgenic monocot plant comprising: 
 (a) constructing a (TPSP) fusion gene sequence consisting of a fused trehalose-6-phosphate synthetase (TPS) gene and trehalose-6-phosphate phosphatase (TPP) gene;    (b) transforming said TSPA fused gene sequence into a recipient plant cell; and    (c) regenerating said plant cell into a mature plant, wherein said mature plant is a transgenic monocot plant comprising said (TPSP) fusion gene sequence.    
     
     
         8 . The method of  claim 7  wherein the transgenic monocot plant is selected from the group of monocot plants consisting of; rice, wheat, barley or maize.  
     
     
         9 . A transgenic monocot plant produced by the method of  claim 7.