Microarray substrate, method of use, and products comprising the microarray substrate

Abstract

Provided are a microarray substrate comprising a solid substrate coated with a chemical having a functional residue represented by Formula 1 or 2 below, a method of analyzing a biomolecule using the microarray substrate, and a lab-on-a-chip comprising the microarray substrate: wherein n, the structure within brackets [ ], R 1 , R 2 , R 3 , R 10 , n and I are as defined in the specification.

1 . A microarray substrate comprising a solid substrate coated with a composition comprising a functional residue represented by Formula 1:  
       
         
           
           
               
               
           
         
         wherein,  
         n is an integer of 1 to 10;  
         the structure within brackets [ ] represents a moiety formed by reaction of a tetracarboxylic acid anhydride with an amine to produce —NH(CH 2 ) n NH 2  and with an amino group to produce —NH(CH 2 ) n NH— at opposite sides of the tetracarboxylic acid anhydride;  
         wherein the tetracarboxylic acid anhydride is selected from the group consisting of pyromellitic dianhydride, 3,3′,4,4′-biphenyltetracarboxylic dianhydride, 2,2′,3,3′-biphenyltetracarboxylic dianhydride, 2,3,3′,4′-biphenyltetracarboxylic dianhydride, 1,2,4,5-benzenetetracarboxylic dianhydride, 3,3′,4,4′-benzophenonetetracarboxylic dianhydride, 2,2′,3,3′-benzophenonetetracarboxylic dianhydride, 2,3,3′,4′-benzophenonetetracarboxylic dianhydride, bis(3,4-dicarboxyphenyl)ether dianhydride, bis(3,4-dicarboxyphenyl)sulfone dianhydride, 1,4,5,8-naphthalenetetracarboxylic dianhydride, 1,2,5,6-naphthalenetetracarboxylic dianhydride, 2,3,6,7-naphthalenetetracarboxylic dianhydride, 2,2-bis(3,4-dicarboxyphenyl)-hexafluoropropane dianhydride, cyclobutanetetracarboxylic dianhydride, methylcyclobutanetetracarboxylic dianhydride, and 1,2,3,4-tetracarboxybutane dianhydride; and  
         R 10  is determined by the selected tetracarboxylic acid anhydride.  
       
     
     
         2 . The microarray substrate of  claim 1 , wherein the functional residue is represented by Formula 3:  
       
         
           
           
               
               
           
         
         wherein,  
         n is an integer of 1 to 10.  
       
     
     
         3 . The microarray substrate of  claim 2 , wherein n is 2.  
     
     
         4 . A microarray substrate comprising a solid substrate coated with a composition comprising a functional residue represented by Formula 2:  
       
         
           
           
               
               
           
         
         wherein,  
         R 1  is —OH or —NH(CH 2 ) n NH 2 ;  
         R 2  is —OH or —NH(CH 2 ) n NH 2 ;  
         n is an integer of 1 to 10;  
         R 3  is C 110  alkyl; and  
         I is an integer of 1 to 30,000.  
       
     
     
         5 . The microarray substrate of  claim 4 , wherein the functional residue is represented by Formula 4:  
       
         
           
           
               
               
           
         
         wherein m is an integer of 1 to 10.  
       
     
     
         6 . The microarray substrate of  claim 5 , wherein m is 2.  
     
     
         7 . The microarray substrate of  claim 5 , wherein a ratio of —OH group to —NH(CH 2 ) n NH 2  group is about 2 to 1.  
     
     
         8 . The microarray substrate of  claim 1 , wherein the solid substrate is made of a material selected from the group consisting of silicone, glass, and a plastic material.  
     
     
         9 . The microarray substrate of  claim 4 , wherein the solid substrate is made of a material selected from the group consisting of silicone, glass, and a plastic material.  
     
     
         10 . A method for analyzing a target biomolecule, comprising: 
 obtaining a target biomolecule; and    hybridizing a probe biomolecule, immobilized on the microarray substrate of  claim 1  at a first pH, and the target biomolecule at a second pH.    
     
     
         11 . The method of  claim 10 , wherein the functional residue is represented by Formula 3 below:  
       
         
           
           
               
               
           
         
         wherein n is an integer of 1 to 10.  
       
     
     
         12 . The method of  claim 11 , wherein n is 2.  
     
     
         13 . The method of  claim 10 , wherein the first pH is about 2 to about 3.5, the second pH is about 5 to about 10.  
     
     
         14 . The method of  claim 10 , wherein the solid substrate is made of a material selected from a group consisting of silicone, glass, and a plastic material.  
     
     
         15 . The method of  claim 10 , wherein the biomolecule is a nucleic acid.  
     
     
         16 . A method of analyzing a target biomolecule, comprising: 
 obtaining a target biomolecule; and    hybridizing a probe biomolecule, immobilized on the microarray substrate of  claim 4  at a first pH, and the target biomolecule at the second pH.    
     
     
         17 . The method of  claim 16  wherein the functional residue is represented by Formula 4 below:  
       
         
           
           
               
               
           
         
         wherein, wherein m is an integer of 1 to 10.  
       
     
     
         18 . The method of  claim 17 , wherein m is 2.  
     
     
         19 . The method of  claim 17 , wherein a ratio of —OH group to —NH(CH 2 ) n NH 2  group is about 2 to 1.  
     
     
         20 . The method of  claim 16 , wherein the first pH is about 2 to about 3.5, and the second pH is about 5 to about 10.  
     
     
         21 . The method of  claim 16 , wherein the solid substrate is made of a material selected from the group consisting of silicone, glass, and a plastic material.  
     
     
         22 . The method of  claim 16 , wherein the biomolecule is a nucleic acid.  
     
     
         23 . A lab-on-a-chip comprising the microarray substrate of  claim 1 .  
     
     
         24 . A lab-on-a-chip comprising the microarray substrate of  claim 4 .  
     
     
         25 . A method of manufacturing a microarray substrate, comprising 
 immobilizing a tetracarboxylic acid anhydride on a solid substrate coated with an amino group: and    reacting the tetracarboxylic acid anhydride with an amine compound represented by NH 2 (CH 2 ) n NH 2 , wherein n is an integer of 1 to 10.    
     
     
         26 . A method of manufacturing a microarray substrate, comprising 
 immobilizing a compound represented by Formula 5 on an amino group-coated substrate; and    reacting the compound with an amine compound represented by NH 2 (CH 2 ) n NH 2 , wherein n is an integer of 1 to 10;                          wherein, R 3  is C 1-10  alkyl; and    I is an integer of 1 to 30,000.