Process and substances for the release of a growth-regulating factor from endothelial cells

Abstract

A method of stimulating endothelial cells cultivated in a nutrient medium to release a growth-factor into the nutrient medium by addition of at least one pentacyclic oxindole alkaloid into the nutrient medium.

1 . A method of inducing endothelial cells to release a growth-factor that is able to regulate proliferation of lymphocytes, said method comprising stimulating said endothelial cells with at least one pentacyclic oxindole alkaloid.  
   
   
       2 . The method according to  claim 1 , wherein said at least one pentacyclic oxindole alkaloid is selected from the group consisting of pteropodine, isopteropodine, speciophylline, uncarine F, mitraphylline and isomitraphylline.  
   
   
       3 . The method according to  claim 1 , wherein said endothelial cells are in a living subject and said at least one pentacyclic oxindole alkaloid is administered to the living subject.  
   
   
       4 . The method according to  claim 1 , wherein said endothelial cells are cultivated in a nutrient medium containing said at least one pentacyclic oxindole alkaloid.  
   
   
       5 . A method of producing a preparation containing a growth-factor that is able to regulate proliferation of lymphocytes, said method comprising stimulating endothelial cells with at least one pentacyclic oxindole alkaloid, whereby said endothelial cells are stimulated to release said growth-factor, and removing the endothelial cells.  
   
   
       6 . The method according to  claim 5 , wherein, when said endothelial cells are cultivated in a nutrient medium, said at least one pentacyclic oxindole alkaloid is added to said nutrient medium to induce a release of said growth-factor into said nutrient medium, and removing the endothelial cells from said nutrient medium.  
   
   
       7 . The method according to  claim 5 , wherein said at least one pentacyclic oxindole alkaloid is selected from the group consisting of pteropodine, isopteropodine, speciophylline, uncarine F, mitraphylline and isomitraphylline.