Fibrinogen from transgenic animals

Abstract

The present invention provides a method for the part purification of fibrinogen from milk, the method comprising the transfer of protease enzyme which is present in the milk, into the whey phase with the removal or partition of fibrinogen into another phase of the milk. The present invention also provides a method for obtaining fibrinogen from a fluid, the method comprising: a) contacting the fluid with a hydrophobic interaction chromatography resin under conditions where the fibrinogen binds to the resin; and b) removing the bound protein by means of elution.

1 . Transgenic fibrinogen having a predetermined F1 fragment to F2 fragment ratio, obtainable from milk, at least partly purified, having at least one of improved stability or increased integrity of the fibrinogen alpha chain.  
   
   
       2 . The transgenic fibrinogen of  claim 1  having a pre-selected A chain integrity.  
   
   
       3 . The transgenic fibrinogen of  claim 1 , wherein the fibrinogen comprises at least 80% F1 fibrinogen.  
   
   
       4 . The transgenic fibrinogen of any of  claims 1  to  3 , substantially free from viral contamination.  
   
   
       5 . The transgenic fibrinogen of any of  claims 1  to  3 , comprising fibrinogen 1, fibrinogen 2, or a combination thereof.  
   
   
       6 . The transgenic fibrinogen of  claim 1 , obtainable by a method of part purification of fibrinogen having a high Aα-chain integrity from milk, the method comprising the following steps: 
 a) precipitating the fibrinogen from milk;    b) separating the precipitated fibrinogen from protease enzymes contained in whey and thereby recovering a part-purified fibrinogen, wherein said part-purified fibrinogen comprises a high and low molecular weight sub-fractions;    c) contacting the part-purified fibrinogen with a hydrophobic interaction chromatography resin under conditions wherein the fibrinogen binds to the resin; and    d) removing the bound fibrinogen by means of elution wherein elution results in the selective removal of said fibrinogen sub-fractions to produce high Aα-chain integrity fibrinogen.